A new enzyme immunoassay for PCDD/F TEQ screening in environmental samples: Comparison to micro-EROD assay and to chemical analysis

• Published in: Chemosphere (Oxford) Vol. 38; no. 14; pp. 3313 - 3318
• Main Authors: Li, W., Wu, W.Z., Barbara, R.B., Schramm, K.-W., Kettrup, A.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.06.1999; Elsevier
• Subjects: Animal, plant and microbial ecology; Applied ecology; Applied sciences; Benzofurans - analysis; Biological and medical sciences; Brackish; Cytochrome P-450 CYP1A1 - metabolism; Ecotoxicology, biological effects of pollution; Environmental Monitoring - methods; Enzyme ImmunoAssay; EROD; Exact sciences and technology; Freshwater; Fundamental and applied biological sciences. Psychology; Global environmental pollution; Immunoenzyme Techniques - methods; Immunoenzyme Techniques - standards; Marine; PCDD/F; Pollution; Polychlorinated Dibenzodioxins - analogs & derivatives; Polychlorinated Dibenzodioxins - analysis; Sensitivity and Specificity; Soil Pollutants - analysis; Techniques; TEQ; TEQ; PCDD/F; EROD; Enzyme ImmunoAssay; Chemical analysis; Bioassay; Wood; Ecotoxicology; Toxicity; Enzyme immunoassay; Sediments; Fly ash; Pollution; Investigation method; Organochlorine compounds; Chlorine Organic compounds; Content; Toxic equivalency factor; Environment; Organic compounds
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/S0045-6535(98)00563-3

A new Enzyme ImmunoAssay (EIA) for PCDD/F TEQ measurement in extracts of environmental samples was described. The bioassay TEQ which derived from EIA and EROD were compared with each other and with results from chemical analysis. For all environmental samples, the EROD-TEQ is higher than the value from chemical analysis. However, the EIA-TEQ is much more identical with the value from chemical analysis. Our results indicate that the EIA assay is a complementary method to the EROD assay and should be useful as a rapid and sensitive screening tool for environmental samples in many situations.