Isolation and structural studies of the neutral oligosaccharide units from bovine glycophorin

• Published in: Biochimica et Biophysica Acta (BBA) - General Subjects Vol. 677; no. 3; pp. 462 - 470
• Main Authors: Fukuda, K, Tomita, M, Hamada, A
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 05.11.1981; Elsevier BV
• Subjects: Animals; Borohydrides; Bovine erthrocyte; Carbohydrate Conformation; Carbohydrates; Carbohydrates - analysis; Cattle; Chemical Phenomena; Chemistry; Chromatography, Gel; Chromium; Chromium Compounds; Glycophorin; Glycophorin - analysis; Glycophorins; Hydrolysis; Methylation; Oligosaccharide structure; Oligosaccharides; Oligosaccharides - isolation & purification; Oxides; Sialoglycoproteins; Sialoglycoproteins - analysis; Oligosaccharide structure; Glycophorin; Bovine erthrocyte
• ISSN: 0304-4165; 0006-3002; 1872-8006
• DOI: 10.1016/0304-4165(81)90260-9

The O-glycosidically linked carbohydrate units of glycophorin from bovine erythrocyte membranes were released as reduced oligosaccharides by alkaline borohydride treatment. These oligosaccharides were separated by ion-exchange chromatography followed by gel filtration. Three oligosaccharides, a penta- a hepta- and a decassacharide were obtained as the major components from the neutral fraction, and seven fractions were separated from the acidic fractions. All of the fractions were found to contain galactose and N-acetylglucosamine in variable amounts, as well as N-acetylgalactosaminitol. Studies of the neutral oligosaccharides by methylation analyses, nitrous acid deamination and Amith degradation, indicated the structure of the pentasaccharide to be Gal(1→3)-Gal(1→4)GlcNAc(1→3) Gal(1→3)GalNAcol and that of the heptasaccharide to be Gal91→3)Gal(1→4)-GlcNAc(1→3)Gal(1→4)GlcNAc(1→3)Gal(1→3)GalNAcol. The highest molecular weight fraction, decasaccharide in the neutral fraction had a branching point at C-6 of a galactose residue.