Differential expression of invertase genes in internal and external phloem tissues of potato (Solanum tuberosum L.)

• Published in: Journal of experimental botany Vol. 51; no. 345; pp. 817 - 821
• Main Authors: Hedley, P.E., Maddison, A.L., Davidson, D., Machray, G.C.
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.04.2000; OXFORD UNIVERSITY PRESS
• Subjects: Agronomy. Soil science and plant productions; Artificial Gene Fusion; beta-Fructofuranosidase; Biological and medical sciences; Classical and quantitative genetics. Population genetics. Molecular genetics; Cloning, Molecular; Complementary DNA; Enzymes; Exons; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Regulation, Plant; Generalities. Genetics. Plant material; Genes; Genes, Reporter; Genetics and breeding of economic plants; Glycoside Hydrolases - genetics; Glycoside Hydrolases - metabolism; Histocytochemistry; Molecular and cellular biology; Molecular genetics; Phloem; Plant Structures - anatomy & histology; Plant Structures - enzymology; Plant Structures - genetics; Plants; Plants, Genetically Modified; Polymerase chain reaction; Promoter; Promoter regions; Promoter Regions, Genetic; SHORT COMMUNICATION; Solanum tuberosum - anatomy & histology; Solanum tuberosum - enzymology; Solanum tuberosum - genetics; source‐sink; Transgenic plants; vascular bundle; Vascular bundles; Enzyme; Solanum tuberosum; β-Fructofuranosidase; Tissue specificity; Gene expression; Characterization; Phloem; Vascular bundle; Dicotyledones; Glycosidases; Angiospermae; Hydrolases; Spermatophyta; Tuber plant; Solanaceae; Molecular cloning; O-Glycosidases
• ISSN: 0022-0957; 1460-2431
• DOI: 10.1093/jexbot/51.345.817

The cloning of promoter sequences of two invertase genes from potato (Solanum tuberosum L.) is described. Histochemical analysis of series of reporter transgenic lines reveals phloem‐expression from both promoters, with one expressed preferentially in internal phloem and the other in external phloem of stem vascular bundles.