Evaluation of chitoligosaccharides effect upon probiotic bacteria

The main objective of the present study was to evaluate the antibacterial effect – through the determination of minimum inhibitory (and lethal) concentrations, as well as the possible prebiotic potential of chitooligosaccharides (COS) – through the determination of growth curves, on Bifidobacterium...

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Published inInternational journal of biological macromolecules Vol. 50; no. 1; pp. 148 - 152
Main Authors Fernandes, João C., Eaton, Peter, Franco, Isabel, Ramos, Óscar S., Sousa, Sérgio, Nascimento, Henrique, Gomes, Ana, Santos-Silva, Alice, Xavier Malcata, F., Pintado, Manuela E.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.01.2012
Subjects
Anti-Bacterial Agents - chemistry
antibacterial properties
Atomic force microscopy
bacteria
Bifidobacterium - metabolism
Bifidobacterium animalis
carbon
Carbon - chemistry
cell biology
Chitin - chemistry
Chitooligosaccharides
Chitosan
Culture Media - chemistry
Glucose - chemistry
Hydrogen-Ion Concentration
Lactobacillus acidophilus
Lactobacillus acidophilus - metabolism
Microbial Sensitivity Tests
Microscopy, Atomic Force - methods
nutrient uptake
Oligosaccharides - chemistry
prebiotics
Probiotic bacteria
probiotics
Probiotics - metabolism
Time Factors
Probiotic bacteria
Atomic force microscopy
Chitosan
Chitooligosaccharides
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Summary:The main objective of the present study was to evaluate the antibacterial effect – through the determination of minimum inhibitory (and lethal) concentrations, as well as the possible prebiotic potential of chitooligosaccharides (COS) – through the determination of growth curves, on Bifidobacterium animalis Bb12, Bifidobacterium animalis Bo and Lactobacillus acidophilus Ki. Atomic force microscopy was further used to obtain high resolution images of COS effects upon the cell morphology. Our results demonstrate that COS do not stimulate the growth of those strains, neither the strains are capable of using COS as a primary source of carbon. Analysis of morphology when exposed to inhibitory/bactericidal concentrations, suggested that COS do not exert any direct damage upon the bacteria structure, instead the bacteria are apparently covered by COS, which likely prevent nutrient uptake.
Bibliography:http://dx.doi.org/10.1016/j.ijbiomac.2011.10.011
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0141-8130
1879-0003
1879-0003
DOI:10.1016/j.ijbiomac.2011.10.011