A novel signal-off electrochemiluminescence biosensor for the determination of glucose based on double nanoparticles

• Published in: Biosensors & bioelectronics Vol. 63; pp. 519 - 524
• Main Authors: Liu, Linlin, Ma, Qiang, Li, Yang, Liu, ZiPing, Su, Xingguang
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 15.01.2015; Elsevier
• Subjects: Au nanoparticles; Biological and medical sciences; Biosensing Techniques - instrumentation; Biosensor; Biosensors; Biotechnology; Cadmium tellurides; CdTe QDs; Chitosan; Conductometry - instrumentation; Electrochemiluminescence; Electrodes; Equipment Design; Equipment Failure Analysis; Fundamental and applied biological sciences. Psychology; Glassy carbon; Glucose; Glucose - analysis; Glucose - chemistry; Gold; Gold - chemistry; Luminescent Measurements - instrumentation; Metal Nanoparticles - chemistry; Methods. Procedures. Technologies; Nanoparticles; Quantum Dots; Various methods and equipments; Au nanoparticles; Electrochemiluminescence; Chitosan; Glucose; Biosensor; CdTe QDs; Nanoparticle
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2014.07.087

In this work, a novel facile signal-off electrochemiluminescence (ECL) biosensor has been developed for the determination of glucose based on the integration of chitosan (CHIT), CdTe quantum dots (CdTe QDs) and Au nanoparticles (Au NPs) on the glassy carbon electrode (GCE). Chitosan displays high water permeability, hydrophilic property, strong hydrogel ability and good adhesion to load the double nanoparticles to the glassy carbon electrode surfaces. Au NPs are efficient glucose oxidase (GOx)-mimickess to catalytically oxidize glucose, similar to the natural process. Upon the addition of glucose, the Au NPs catalyzed glucose to produce gluconic acid and hydrogen peroxide (H2O2) based on the consumption of dissolved oxygen (O2), which resulted in a quenching effect on the ECL emission. Therefore, the determination of glucose could be achieved by monitoring the signal-off ECL biosensor. Under the optimum conditions, the ECL intensity of CdTe QDs and the concentration of glucose have a good linear relationship in the range of 0.01–10mmolL−1. The limit of detection for glucose was 5.28μmolL−1 (S/N=3). The biosensor showed good sensitivity, selectivity, reproducibility and stability. The proposed biosensor has been employed for the detection of glucose in human serum samples with satisfactory results. •A novel signal-off ECL biosensor has been developed for glucose detection.•The biosensor shows good sensitivity, reproducibility and stability.•The biosensor provided a convenient, low-cost and specific method for glucose detection.