Immune response and stromal changes in ductal carcinoma in situ of the breast are subtype dependent

• Published in: Modern pathology Vol. 33; no. 9; pp. 1773 - 1782
• Main Authors: Agahozo, Marie Colombe, Westenend, Pieter J., van Bockstal, Mieke R., Hansum, Tim, Giang, Jenny, Matlung, Sanneke E., van Deurzen, Carolien H.M.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.09.2020; Elsevier Limited
• Subjects: Adult; Aged; Aged, 80 and over; Biomarkers, Tumor; Breast - immunology; Breast - metabolism; Breast - pathology; Breast cancer; Breast Neoplasms - immunology; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; Carcinoma, Intraductal, Noninfiltrating - immunology; Carcinoma, Intraductal, Noninfiltrating - metabolism; Carcinoma, Intraductal, Noninfiltrating - pathology; CD20 antigen; CD4 antigen; CD8 antigen; ErbB-2 protein; Female; Foxp3 protein; Humans; Immunohistochemistry; Invasiveness; Lymphocytes; Lymphocytes T; Lymphocytes, Tumor-Infiltrating - immunology; Lymphocytes, Tumor-Infiltrating - pathology; Macrophages; Middle Aged; Receptor, ErbB-2 - metabolism; Receptors, Estrogen - metabolism; Receptors, Progesterone - metabolism; Stromal Cells - immunology; Stromal Cells - pathology; Tumor Microenvironment - immunology
• ISSN: 0893-3952; 1530-0285
• DOI: 10.1038/s41379-020-0553-9

Ductal carcinoma in situ (DCIS) associated stromal changes and influx of immune cells might be mediators of progression to invasive breast cancer. We studied the interaction between DCIS-associated stromal changes, and immune cell distribution and composition in a well-characterized patient cohort. We included 472 patients with DCIS. The presence of stromal changes, signs of regression, and DCIS-associated immune cell position were determined on hematoxylin and eosin-stained slides. Immune cell composition was characterized by immunohistochemistry (CD4, CD8, CD20, CD68, and FOXP3). The number of intraductal immune cells was quantified per mm2. The interaction between stromal changes, signs of DCIS regression, immune cell composition and location was explored. Stromal changes and signs of DCIS regression were identified in 30 and 7% of the patients, respectively. Intraductal immune cells mainly comprised CD68+ macrophages and CD8+ T cells. Patients with stromal changes had significantly less influx of immune cells within the duct. DCIS regression was associated with an increased number of intraductal FOXP3+ T cells. The highest number of intraductal CD8+ T cells was seen in the ER+ HER2+ subtype. We suggest that DCIS-associated stromal changes prevent the interaction between immune cells and DCIS cells. However, in case of DCIS regression, we surmise a direct interaction between DCIS cells and immune cells, in particular FOXP3+ cells. Furthermore, the increased number of intraductal CD8+ T cells in the ER+ HER2+ DCIS subtype suggests a subtype-specific immune response, which is likely to play a role in the distinct biological behavior of different DCIS subtypes.