Oocyte Cryopreservation in Domestic Animals and Humans: Principles, Techniques and Updated Outcomes

• Published in: Animals (Basel) Vol. 11; no. 10; p. 2949
• Main Authors: Tharasanit, Theerawat, Thuwanut, Paweena
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 13.10.2021; MDPI
• Subjects: animal; Animals; Apoptosis; Cell viability; Cooling; Cryoinjury; Cryopreservation; Cytoplasm; Domestic animals; Embryos; Fertility; Freezing; Gametes; Gametocytes; human; In vitro fertilization; Infertility; Laboratories; Molecular weight; oocyte; Oocytes; Ovaries; Permeability; Physiology; Pregnancy; Principles; Reproductive technologies; Reproductive technology; Review; Success; Technology
• ISSN: 2076-2615; 2076-2615
• DOI: 10.3390/ani11102949

Oocyte cryopreservation plays important roles in basic research and the application of models for genetic preservation and in clinical situations. This technology provides long-term storage of gametes for genetic banking and subsequent use with other assisted reproductive technologies. Until recently, oocytes have remained the most difficult cell type to freeze, as the oocytes per se are large with limited surface area to cytoplasm ratio. They are also highly sensitive to damage during cryopreservation, and therefore the success rate of oocyte cryopreservation is generally poor when compared to noncryopreserved oocytes. Although advancement in oocyte cryopreservation has progressed rapidly for decades, the improvement of cryosurvival and clinical outcomes is still required. This review focuses on the principles, techniques, outcomes and prospects of oocyte cryopreservation in domestic animals and humans.