Taenia solium: characterization of a small heat shock protein (Tsol-sHSP35.6) and its possible relevance to the diagnosis and pathogenesis of neurocysticercosis

• Published in: Experimental parasitology Vol. 110; no. 1; pp. 1 - 11
• Main Authors: Ferrer, Elizabeth, González, Luis Miguel, MildredFoster-Cuevas, Cortéz, Maria Milagros, Dávila, Iris, Rodríguez, Mercedes, Sciutto, Edda, Harrison, Leslie J.S., Parkhouse, R. Michael E., Gárate, Teresa
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.05.2005; Elsevier
• Subjects: Amino Acid Sequence; amino acid sequences; Animals; Antibodies, Helminth - blood; Antibodies, Helminth - immunology; antibody detection; antigen detection; Antigens, Helminth - chemistry; Antigens, Helminth - genetics; Antigens, Helminth - immunology; Base Sequence; Biological and medical sciences; Blotting, Western; Central nervous system; Cerebrospinal fluid; Cestode; CNS; complementary DNA; CSF; cysticercosis; Diagnosis; DNA, Complementary - chemistry; DNA, Complementary - isolation & purification; E/S; Electrophoresis, Polyacrylamide Gel; ELISA; Enzyme-Linked Immunosorbent Assay; Excretory/secretory antigen; Fundamental and applied biological sciences. Psychology; heat shock proteins; Heat-Shock Proteins - chemistry; Heat-Shock Proteins - genetics; Heat-Shock Proteins - immunology; Humans; Immune Sera - immunology; Isoelectric Point; Life cycle. Host-agent relationship. Pathogenesis; Metacestode; Molecular Sequence Data; Molecular Weight; NCC; Neurocysticercosis; Neurocysticercosis - diagnosis; Neurocysticercosis - parasitology; nucleotide sequences; Open Reading Frames - genetics; Pathogenesis; Protozoa; Rabbits; Recombinant antigen; Recombinant Proteins - chemistry; Recombinant Proteins - genetics; Recombinant Proteins - immunology; Sensitivity and Specificity; Sequence Alignment; Sequence Homology, Amino Acid; serodiagnosis; sHSP; Small heat shock protein; Swine; Taenia solium; Taenia solium - chemistry; Taenia solium - genetics; Taenia solium - immunology; Pathogenesis; Recombinant antigen; sHSP; Cestode; E/S; CNS; Small heat shock protein; Taenia solium; Metacestode; NCC; CSF; Diagnosis; ELISA; Enzyme; Central nervous system; Plathelmintha; Parasite; Central nervous system; CSF; Cestoda; Neurocysticercosis; CNS; Antigen; Characterization; Small heat shock protein; NCC; Heat shock protein; Helmintha; Cerebrospinal fluid; E/S; Invertebrata; Enzyme-linked immunosorbent assay; Metacestode; Cestode; Small heat shock protein; Recombinant antigen; Diagnosis; Pathogenesis; sHSP; Excretory/secretory antigen; ELISA
• ISSN: 0014-4894; 1090-2449
• DOI: 10.1016/j.exppara.2004.11.014

A cDNA encoding for a predicted small heat shock protein (sHSP), Tsol-sHSP35.6, has been isolated by antibody screening of a Taenia solium c-DNA library. The clone was a full-length sequence (1172 bp) with an open reading frame of 945 bp and encoded for a 314 amino acid protein with deduced molecular mass of 35.6 kDa, isoelectric point of 5.6 and the characteristic HSP20/α-crystallin domain duplicated. It was highly conserved, with a high sequence similarity with other platyhelminth sHSPs. Western blot analysis, using serum from neurocysticercosis patients (NCC), indicated that the purified Tsol-sHSP35.6 expression product was immunogenic, while in indirect ELISA, using the purified Tsol-sHSP35.6 expression product as antigen and serum samples from pigs and humans, 80% of T. solium infected pigs and 84% of patients with active, or 71% of patients with inactive NCC were sero-positive. The possible relevance of Tsol-sHSP35.6 in the diagnosis and pathogenesis of NCC is discussed.