Identification of European-type hepatitis E virus subtype 3e isolates in Japanese wild boars: Molecular tracing of HEV from swine to wild boars

• Published in: Infection, genetics and evolution Vol. 18; pp. 287 - 298
• Main Authors: Nakano, Tatsunori, Takahashi, Kazuaki, Arai, Masahiro, Okano, Hiroshi, Kato, Hideaki, Ayada, Minoru, Okamoto, Hiroaki, Mishiro, Shunji
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.08.2013
• Subjects: Animals; Animals, Wild; blood serum; Cluster Analysis; gene flow; genome; Genotype; Hepatitis E - veterinary; Hepatitis E - virology; Hepatitis E virus; Hepatitis E virus - classification; Hepatitis E virus - genetics; Japan; nucleotide sequences; Phylogenetic analysis; Phylogeny; Phylogeography; Subtype 3e; Sus scrofa; Swine; Swine Diseases - virology; Wild boar; wild boars; Europe; Japan; Phylogenetic analysis; BF; TMRCA; Japan; Subtype 3e; Wild boar; HEV; MCMC; Genotype; Hepatitis E virus; Bayes factor; time of the most recent common ancestor; Markov chain Monte Carlo
• ISSN: 1567-1348; 1567-7257
• DOI: 10.1016/j.meegid.2013.06.004

•We identified the indigenization of HEV subtype 3e isolates in Japanese wild boars.•HEV subtype 3e isolates were recovered from wild animal outside Europe.•The phylogenetic analyses suggested the ancestor of the isolates came from Europe.•Our analyses suggest the gene flow of subtype 3e isolates from swine to wild boars.•Molecular evidence of swine-to-boar HEV transmission in the past in Japan. Nucleotide sequences of hepatitis E virus (HEV) isolates infecting wild boars in Mie prefecture, which is located in the central region of Japan and is far from the most prevalent regions of HEV infection in Japan, were determined and characterised. Among 144 serum samples of wild boars captured in Mie prefecture, 7 were positive for HEV-RNA. The nucleotide sequence of nearly the entire genome was determined for 4 of the 7 positive samples. Phylogenetic tree analyses indicated that 6 samples were subtype 3e and 1 was subtype 3a among the 7 isolates. We identified the indigenization of subtype 3e isolates in Japanese wild boars. Furthermore, 5 subtype 3e isolates were closely related and were located in the peripheral branch of subtype 3e isolates from European countries in the phylogenetic tree. The structure indicated that the ancestor of the 5 subtype 3e isolates originated in Europe. The phylogenetic structure and coalescent analyses suggested that the subtype 3e isolates entered Japan from Europe by importation of large-race pigs around 1966. The results also indicated that several lineages of subtype 3e expanded to a wide area of Japan around 1992 and 1 of the lineages was indigenized in wild boars in Mie prefecture between 1992 and 2009. The appearance of a wild boar cluster in the peripheral branch in the phylogenetic lineage may indicate the direction of gene flow of HEV subtype 3e from swine to wild boars. Clarification of the transmission direction or route should be helpful to prevent a future endemic or epidemic of HEV infection.