Real-time label-free immunoassay of interferon-gamma and prostate-specific antigen using a Fiber-Optic Localized Surface Plasmon Resonance sensor

• Published in: Biosensors & bioelectronics Vol. 39; no. 1; pp. 346 - 351
• Main Authors: Jeong, Hyeon-Ho, Erdene, Norov, Park, Jae-Hyoung, Jeong, Dae-Hong, Lee, Ho-Young, Lee, Seung-Ki
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 15.01.2013; Elsevier
• Subjects: Antibodies, Immobilized - immunology; Antigen-Antibody Reactions; Biological and medical sciences; Biotechnology; Equipment Design; Fiber-Optic Localized Surface Plasmon Resonance (FO LSPR); Fiber-optic sensor; Fundamental and applied biological sciences. Psychology; Gold - chemistry; Humans; Immunoassay - instrumentation; Interferon-gamma (IFN-γ); Interferon-gamma - analysis; Interferon-gamma - immunology; Label-free immunoassay; Limit of Detection; Male; Nanoparticles - chemistry; Nanoparticles - ultrastructure; Prostate-specific antigen (PSA); Prostate-Specific Antigen - analysis; Prostate-Specific Antigen - immunology; Real-time detection; Surface Plasmon Resonance - instrumentation; Prostate-specific antigen (PSA); Fiber-Optic Localized Surface Plasmon Resonance (FO LSPR); Interferon-gamma (IFN-γ); Fiber-optic sensor; Real-time detection; Label-free immunoassay; Fiber-Optic Localized Surface Plasmon; Cytokine; Fiber; Optics; Surface plasmon; Real time; Immunological method; Prostate specific antigen; Detector; Resonance (FO LSPR); Resonance; Detection; Gamma interferon
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2012.08.013

A Fiber-Optic Localized Surface Plasmon Resonance (FO LSPR) sensor was fabricated using spherical gold nanoparticles (Au NPs) on a flattened end-face of the optical fiber. The Au NPs were easily synthesized by the Turkevich method and were immobilized on the end-face of the optical fiber by using a self-assembled monolayer (SAM). In order to examine the possibility of its application as a biosensor for label-free immunoassays, the fabricated FO LSPR sensor was used for the detection of the antibody–antigen reaction of interferon-gamma (IFN-γ) and the limit of detection (LOD) was approximately 2pg/ml. Herein, The antibodies and bovine serum albumins (BSAs) were immobilized on the Au NPs by physisorption. Also, the FO LSPR sensor was used for the detection of a prostate-specific antigen (PSA) and the LOD was 1pg/ml below. The fabricated FO LSPR sensor can be used for real-time label-free immunoassay having fast detection time, high resolution and sensitivity. In addition, the proposed sensor platform has the advantages of low cost, simple optical setup, remote sensing, simple fabrication, real-time detection, low sample volume, and potential application to in-vivo detection systems. ► A biosensor was developed by using a Fiber-Optic Localized Surface Plasmon Resonance. ► The sensor can rapidly detect the antibody–antigen reaction of an interferon-gamma. ► It also can detect a prostate-specific antigen with high resolution and sensitivity. ► The biosensor can be applied for clinical testing.