GABA (A) receptor subunits RNA expression in mice peritoneal macrophages modulate their IL-6/IL-12 production

• Published in: Journal of neuroimmunology Vol. 188; no. 1; pp. 64 - 68
• Main Authors: Reyes-García, Maria Guadalupe, Hernández-Hernández, Francisca, Hernández-Téllez, Beatriz, García-Tamayo, Fernando
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.08.2007
• Subjects: Allergy and Immunology; Analysis of Variance; Animals; Dose-Response Relationship, Drug; Drug Interactions; GABA; GABA A receptor; GABA Antagonists - pharmacology; gamma-Aminobutyric Acid - metabolism; IL-12; IL-6; Interleukin-12 - metabolism; Interleukin-6 - metabolism; Macrophages; Macrophages - metabolism; Male; Mice; Mice, Inbred BALB C; Neurology; Peritoneum - cytology; Picrotoxin - pharmacology; Protein Subunits - genetics; Receptors, GABA-A - genetics; Receptors, GABA-A - metabolism; RNA, Messenger - metabolism; RNA, Messenger - physiology; RT-PCR; GABA; GABA A receptor; Macrophages; IL-12; RT-PCR; IL-6
• ISSN: 0165-5728; 1872-8421
• DOI: 10.1016/j.jneuroim.2007.05.013

Abstract The expression and functionality of the gamma aminobutyric acid A receptor (GABAA R) in mice macrophages was explored. Reverse Transcriptase-Polymerase Chain Reaction showed that macrophages express the GABAA R RNA for the α1, α2, β3 and δ subunits, but not for the α5, β1, β2 and γ3 subunits. The expression of α1 subunit was also revealed by confocal microscopy. LPS-stimulated macrophages significantly reduced their in vitro production of IL-6 and IL-12 after GABA adding to the culture medium. These results showed that BALB/c mice peritoneal macrophages express a functional subset of GABAA R subunits.