High levels of fibronectin in the stroma of aural cholesteatoma

• Published in: American journal of otolaryngology Vol. 16; no. 4; pp. 232 - 235
• Main Authors: Schilling, Volker, Holly, Anja, Bujía, Jesus, Schulz, Peter, Kastenbauer, Ernst
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.07.1995; Elsevier
• Subjects: Antibodies, Monoclonal; Biological and medical sciences; Cholesteatoma, Middle Ear - physiopathology; Ear, Middle - physiopathology; Ear, Middle - ultrastructure; Ent. Stomatology; Epidermis - ultrastructure; Epithelium - ultrastructure; Fibronectins - analysis; Humans; Immunohistochemistry; Investigative techniques, diagnostic techniques (general aspects); Medical sciences; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; T-Lymphocytes; Immunohistochemistry; Pathology; Oral cavity; Cholesteatoma; Stomatology; Stroma; Exploration; Fibronectin
• ISSN: 0196-0709; 1532-818X
• DOI: 10.1016/0196-0709(95)90148-5

Purpose: Because abundant fibronectin deposition is a hallmark of healing cutaneous wounds and provides a matrix for hyperproliferative and migratory epidermal cells, the distribution of fibronectin in aural cholesteatoma was investigated immunohistochemical ly. Materials and Methods: A monoclonal antibody against the major cell binding domain of human fibronectin was used to stain 4-μm cryosections of cholesteatoma tissue by the alkaline phospatase-antialkaline phosphatase method. Section of normal retroauricular skin served as control. Results: When processed in parallel, fibronectin staining was much stronger in the stroma of cholesteatoma than in normal dermis. The squamous epithelium of both tissues did not show any staining for fibronectin. Conclusions: These observations lend support to the view that the growth of cholesteatoma epithelium reflects an aberrant regenerative process.