Microwave irradiation and cross-linking of collagen

• Published in: Biomaterials Vol. 13; no. 1; pp. 34 - 37
• Main Authors: Visser, C.E., Voute, A.B.E., Oosting, J., Boon, M.E., Kok, L.P.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 1992; Elsevier Science
• Subjects: Animals; Biocompatible Materials; Biological and medical sciences; collagen; Collagen - chemistry; Collagen - radiation effects; Cross-Linking Reagents; Glutaral; Glutaraldehyde fixation; In Vitro Techniques; Materials Testing; Medical sciences; microwave irradiation; Microwaves; Miscellaneous; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; collagen; Glutaraldehyde fixation; microwave irradiation; Fixation; Retraction; Implant; Surgery; Collagen; Microwave irradiation; Crosslinking; Biomaterial; Skin; Glutaral; Biomedical engineering
• ISSN: 0142-9612; 1878-5905
• DOI: 10.1016/0142-9612(92)90092-3

In a multifactorial experiment, dermal sheep collagen was treated in diluted glutaraldehyde solutions, 70% ethyl alcohol, Cialit 1:5000, and distilled water for 1, 3 and 5 min, respectively, in combination with microwave irradiation at different temperature settings. The shrinkage temperature indicating the degree of cross-linking achieved was then determined. Treatment in 0.65% glutaraldehyde with microwave irradiation setting for 60°C resulted in the maximum shrinkage temperature within 1 min, whilst at the lower setting of 50°C the maximum shrinkage temperature for both glutaraldehyde solutions is only reached after 5 min. Neither microwave irradiation by itself, Cialit or ethyl alcohol induce cross-linking of collagen fibres. These findings are relevant for implant studies.