Separation of basic drug substances of very similar structure using micellar electrokinetic chromatography

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 13; no. 4; pp. 489 - 495
• Main Authors: Hansen, Steen Honoré, Bjørnsdottir, Inga, Tjørnelund, Jette
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 01.04.1995; Elsevier Science
• Subjects: Amines - chemistry; amitriptyline; Analysis; basic drugs; Biological and medical sciences; Chromatography; Electrochemistry; General pharmacology; imipramine; Medical sciences; Micellar electrokinetic chromatography (MEKC); Micelles; Molecular Weight; Pharmaceutical Preparations - chemistry; Pharmaceutical Preparations - isolation & purification; Pharmacology. Drug treatments; Surface-Active Agents - chemistry; Tween 20, 3-(N,N-dimethylmyristylammonium) propanesulphonate; Micellar electrokinetic chromatography (MEKC); amitriptyline; Tween 20, 3-(N,N-dimethylmyristylammonium) propanesulphonate; basic drugs; imipramine; Molecules; Separation; Similarity; MECC chromatography; Active ingredient; Tricyclic compound; Base; Quantitative analysis
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/0731-7085(95)01253-H

Small molecules having very similar molecular structure — some even with the same mass over charge — are not trivial to separate by capillary electrophoresis in free solution. However, addition of surfactants to the electrophoretic buffer and thus using the principle of micellar electrokinetic chromatography may provide separation with high resolution. The use of zwitterionic and nonionic surfactants is demonstrated and an example of how a developed system may be used for drug purity testing is given.