Effect of 5-aminolevulinic acid-encapsulate liposomes on photodynamic therapy in human cholangiocarcinoma cells

Intracellular protoporphyrin IX (PpIX) generation following administration of 5-aminolevulinic acid (ALA) has been used in photodynamic therapy (PDT). Subsequent irradiation can lead to selective damage to photosensitizer-treated cells or tissues. In the present work, we describe the enhancement of...

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Published inJournal of nanoscience and nanotechnology Vol. 14; no. 8; p. 5628
Main Authors Choi, Kyung Ha, Chung, Chung-Wook, Kim, Cy Hyun, Kim, Do Hyung, Jeong, Young-Il, Kang, Dae Hwan
Format Journal Article
LanguageEnglish
Published United States 01.08.2014
Subjects
Aminolevulinic Acid - administration & dosage
Aminolevulinic Acid - therapeutic use
Apoptosis
Cell Line, Tumor
Cell Separation
Cholangiocarcinoma - drug therapy
Cholangiocarcinoma - pathology
Flow Cytometry
Humans
Liposomes
Photochemotherapy
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Summary:Intracellular protoporphyrin IX (PpIX) generation following administration of 5-aminolevulinic acid (ALA) has been used in photodynamic therapy (PDT). Subsequent irradiation can lead to selective damage to photosensitizer-treated cells or tissues. In the present work, we describe the enhancement of ALA-induced PpIX accumulation using a liposome carrier. ALA-containing liposomes (Lipo-ALA) were prepared using dipalmitoyl-phosphatidyl choline and in vitro PDT effect was investigated against human cholangiocarcinoma HuCC-T1 cells. Lipo-ALA increased the uptake efficiency into tumor cells compared to ALA itself, which increased the phototoxic effect. A positive relationship was evident between small particle size, PpIX accumulation and cell death after Lipo-ALA based PDT.
ISSN:1533-4880
1533-4899
DOI:10.1166/jnn.2014.8825