Phenotypic and genotypic characteristics of Acacia senegal (L.) Willd. root-nodulating bacteria isolated from soils in the dryland part of Senegal

• Published in: Letters in applied microbiology Vol. 47; no. 2; pp. 85 - 97
• Main Authors: Fall, D, Diouf, D, Ourarhi, M, Faye, A, Abdelmounen, H, Neyra, M, Sylla, S.N, Missbah El Idrissi, M
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.08.2008; Blackwell Publishing Ltd; Blackwell Science
• Subjects: Acacia - microbiology; Acacia senegal; Alphaproteobacteria - classification; Alphaproteobacteria - genetics; Alphaproteobacteria - isolation & purification; Biodiversity; Biological and medical sciences; Cluster Analysis; diversity; DNA Fingerprinting; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; DNA, Ribosomal Spacer - genetics; ecology; Fundamental and applied biological sciences. Psychology; Genes, rRNA; Genotype; genotyping; Mesorhizobium; Microbiology; molecular genetic; Phylogeny; Plant Roots - microbiology; Polymorphism, Restriction Fragment Length; Rhizobium; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; Senegal; Sequence Analysis, DNA; Sequence Homology, Nucleic Acid; Soil Microbiology; stress response symbiosis; Senegal; Symbiosis; Root; stress response symbiosis; Applied microbiology; Diversity; genotyping; Nodulation; Genotype; Ecology; Stress; molecular genetic; Soils; Phenotype; Leguminosae; Dicotyledones; Acacia senegal; Angiospermae; Bacteria; Genetics; Spermatophyta
• ISSN: 0266-8254; 1472-765X
• DOI: 10.1111/j.1472-765X.2008.02389.x

We characterized phenotypically and genotypically root-nodulating bacteria associated with Acacia senegal (L.) Willd. isolated from the soils surrounding A. senegal trees in the dry land area of Senegal. The phenotypical and genotypical characterizations we carried out showed a high diversity of A. senegal root-nodulating bacteria. Phenotypic patterns showed adaptations of the rhizobial strains to many environmental stresses such as heat, drought, and salinity. Twelve molecular groups were distinguished by profiles obtained using polymerase chain reaction/restriction fragment length polymorphism techniques from intergenic spacer region rDNA. The highest genetic diversity was found around the A. senegal rhizosphere. Therefore, A. senegal seemed to have a positive influence on occurrence and genotypical diversity of rhizobial populations. Rhizobial isolates obtained in this study belonged phylogenetically to the genera Mesorhizobium and Rhizobium. Our results provided information about the genetic diversity of the rhizobial strains associated with A. senegal and suggested the adaptability of natural rhizobial populations to major ecological environmental stress within these soil environments. These results suggested a potential selection of compatible and well adapted strains under stress conditions as inoculants for successful A. senegal growth in arid lands.