Optimization of peptic hydrolysis parameters for the production of angiotensin I-converting enzyme inhibitory hydrolysate from Acetes chinensis through Plackett-Burman and response surface methodological approaches

• Published in: Journal of the science of food and agriculture Vol. 92; no. 1; pp. 42 - 48
• Main Authors: Cao, Wenhong, Zhang, Chaohua, Ji, Hongwu, Hao, Jiming
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 15.01.2012; Wiley; John Wiley and Sons, Limited
• Subjects: Acetes chinensis; adverse effects; angiotensin I-converting enzyme inhibitory activity; Angiotensin-Converting Enzyme Inhibitors; Angiotensin-Converting Enzyme Inhibitors - metabolism; Animals; Antihypertensive Agents; Antihypertensive Agents - metabolism; antihypertensive effect; Biological and medical sciences; Blood pressure; Decapoda (Crustacea); Decapoda (Crustacea) - metabolism; Dietary Proteins; Dietary Proteins - metabolism; enzymatic hydrolysis; Enzymes; Food industries; Food science; Fundamental and applied biological sciences. Psychology; Hydrogen-Ion Concentration; hydrolysates; Hydrolysis; metabolism; Peptides; Peptides - metabolism; Physiology; Plackett-Burman design; Protein Hydrolysates; Protein Hydrolysates - metabolism; protein sources; response surface methodology; Shellfish; shrimp; Temperature; angiotensin I-converting enzyme inhibitory activity; Methodology; Enzyme; response surface methodology; enzymatic hydrolysis; Optimization; Design; Hydrolysis; Acetes chinensis; Plackett-Burman design; Hydrolysate; Angiotensin; Production; Response surface; Enzymatic reaction
• ISSN: 0022-5142; 1097-0010; 1097-0010
• DOI: 10.1002/jsfa.4538

BACKGROUND: Angiotensin I‐converting enzyme (ACE) plays an important physiological role in regulating blood pressure. The elevation of blood pressure could be suppressed by inhibiting ACE. ACE inhibitory peptides derived from food proteins could exert antihypertensive effects without side effects. Acetes chinensis is a marine shrimp suitable for the production of ACE inhibitory peptides. The principal objective of this study was to screen for the significant variables, and further to optimize the levels of the selected variables, for the enzymatic production of ACE inhibitory peptides from Acetes chinensis. RESULTS: Plackett–Burman design and response surface methodology were employed to optimize the peptic hydrolysis parameters of Acetes chinensis to obtain a hydrolysate with potent ACE inhibitory activity. The peptic hydrolysis variables were subject to a Plackett–Burman design for screening the main factors. The selected significant parameters such as pH, hydrolysis temperature and enzyme/substrate (E/S) ratio were further optimized using a central composite design. The optimized conditions were: pH 2.5, hydrolysis temperature 45 °C, E/S ratio 17 800 U kg−1 shrimp and substrate concentration 200 g L−1. The results showed that 3–5 h hydrolysis could result in a hydrolysate with ACE inhibition IC50 of 1.17 mg mL−1 and a high DH of 25–27%. CONCLUSION: Plackett–Burman design and RSM performed well in the optimization of peptic hydrolysis parameters of Acetes chinensis to produce hydrolysate with ACE inhibitory activity. A hydrolysate with potent ACE inhibitory activity and high degree of hydrolysis was obtained, so that the yield of ACE inhibitory peptides in it was high. Copyright © 2011 Society of Chemical Industry