Three-dimensional phase contrast angiography of the mouse aortic arch using spiral MRI

• Published in: Magnetic resonance in medicine Vol. 66; no. 5; pp. 1382 - 1390
• Main Authors: Janiczek, Robert L., Blackman, Brett R., Roy, R. Jack, Meyer, Craig H., Acton, Scott T., Epstein, Frederick H.
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.11.2011
• Subjects: Animals; Aorta, Thoracic - anatomy & histology; Humans; Image Enhancement - methods; Imaging, Three-Dimensional - methods; Magnetic Resonance Imaging, Cine - methods; Mathematics; Mice; Mice, Inbred C57BL; Mice, Transgenic; mouse; MRI; phase contrast; Shear Strength; spiral; wall shear stress
• ISSN: 0740-3194; 1522-2594
• DOI: 10.1002/mrm.22937

Atherosclerosis is a complex disease whose spatial distribution is hypothesized to be influenced by the local hemodynamic environment. The use of transgenic mice provides a mechanism to study the relationship between hemodynamic forces, most notably wall shear stress (WSS), and the molecular factors that influence the disease process. Phase contrast MRI using rectilinear trajectories has been used to measure boundary conditions for use in computational fluid dynamic models. However, the unique flow environment of the mouse precludes use of standard imaging techniques in complex, curved flow regions such as the aortic arch. In this article, two‐dimensional and three‐dimensional spiral cine phase contrast sequences are presented that enable measurement of velocity profiles in curved regions of the mouse vasculature. WSS is calculated directly from the spatial velocity gradient, enabling WSS calculation with a minimal set of assumptions. In contrast to the outer radius of the aortic arch, the inner radius has a lower time‐averaged longitudinal WSS (7.06 ± 0.76 dyne/cm2 vs. 18.86 ± 1.27 dyne/cm2; P < 0.01) and higher oscillatory shear index (0.14 ± 0.01 vs. 0.08 ± 0.01; P < 0.01). This finding is in agreement with humans, where WSS is lower and more oscillatory along the inner radius, an atheroprone region, than the outer radius, an atheroprotective region. Magn Reson Med, 2011. © 2011 Wiley Periodicals, Inc.