Gossypium arboreum GHSP26 enhances drought tolerance in Gossypium hirsutum

• Published in: Biotechnology progress Vol. 26; no. 1; pp. 21 - 25
• Main Authors: Maqbool, Asma, Abbas, Waseem, Rao, Abdul Qayyum, Irfan, Muhammad, Zahur, Muzna, Bakhsh, Allah, Riazuddin, Shiekh, Husnain, Tayyab
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.01.2010; Wiley
• Subjects: Biological and medical sciences; Biotechnology; Cauliflower mosaic virus; Chaperones; Cloning, Molecular; Dehydration; Drought resistance; drought stress; Droughts; Expression vectors; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Plant; Genetic Vectors - genetics; genomics; Gossypium; Gossypium - genetics; Gossypium - physiology; Gossypium arboreum; Gossypium hirsutum; heat shock protein; Heat shock proteins; Heat-Shock Proteins, Small - genetics; Heat-Shock Proteins, Small - isolation & purification; Heat-Shock Proteins, Small - metabolism; Integration; Plant viruses; Plants, Genetically Modified; Polymerase chain reaction; Promoter Regions, Genetic - genetics; Promoters; Protein folding; Protein interaction; Protein structure; Stress; Stress, Physiological - genetics; Stress, Physiological - physiology; transformation; Transgenic plants; Malvaceae; Dicotyledones; Angiospermae; Tolerance; Spermatophyta; Gossypium hirsutum; Drought
• ISSN: 8756-7938; 1520-6033; 1520-6033
• DOI: 10.1002/btpr.306

Heat‐shock proteins (HSP) are molecular chaperones for protein molecules. These proteins play an important role in protein–protein interactions such as, folding and assisting in the establishment of proper protein conformation and prevention of unwanted protein aggregation. A small HSP gene GHSP26 present in Gossypium arboreum responds to dehydration. In the present study, an attempt was made to overcome the problem of drought stress in cotton. A cDNA of GHSP26 was isolated from G. arboreum, cloned in plant expression vector, pCAMBIA‐1301 driven by the cauliflower mosaic virus 35S promoter and introduced into Gossypium hirsutum. The integration and expression studies of putative transgenic plants were performed through GUS assay; PCR from genomic DNA, and quantitative real‐time PCR analysis. Transgenic cotton plants showed an enhanced drought tolerance, suggesting that GHSP26 may play a role in plant responsiveness to drought. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2010