Culture of Staphylococcus xylosus in fish processing by-product-based media for lipase production

• Published in: Letters in applied microbiology Vol. 47; no. 6; pp. 549 - 554
• Main Authors: Rebah, F. Ben, Frikha, F, Kamoun, W, Belbahri, L, Gargouri, Y, Miled, N
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.12.2008; Blackwell Publishing Ltd; Blackwell
• Subjects: Animals; Bacterial Proteins - metabolism; Biological and medical sciences; Culture Media - analysis; Culture Media - metabolism; Fish by-products; Fishes; Food-Processing Industry; Fundamental and applied biological sciences. Psychology; growth media; Industrial Waste - analysis; Lipase - metabolism; lipase production; Marine; Microbiology; Sardinops; Staphylococcus - enzymology; Staphylococcus - growth & development; Staphylococcus - metabolism; Staphylococcus xylosus; Thunnus; Waste Management - methods; Staphylococcus xylosus; Enzyme; Growth; Applied microbiology; Triacylglycerol lipase; Esterases; Carboxylic ester hydrolases; lipase production; Production; growth media; Bacteria; Hydrolases; Micrococcales; Fish product; Micrococcaceae; Fish by-products; By product; Culture
• ISSN: 0266-8254; 1472-765X
• DOI: 10.1111/j.1472-765X.2008.02465.x

The objective of this study was to demonstrate that fish-processing by-products could be used as sole raw material to sustain the growth of Staphylococcus xylosus for lipase production. Bacterial growth was tested on supernatants generated by boiling (100°C for 20 min) of tuna, sardine, cuttlefish and shrimp by-products from fish processing industries. Among all samples tested, only supernatants generated from shrimp and cuttlefish by-products sustained the growth of S. xylosus. Shrimp-based medium gave the highest growth (A₆₀₀ = 22) after 22 h of culture and exhibited the maximum lipase activity (28 U ml⁻¹). This effect may be explained by better availability of nutrients, especially, in shrimp by-products. Standard medium (SM) amendments to sardine and tuna by-product-based media stimulated the growth of S. xylosus and the highest A₆₀₀ values were obtained with 75% SM. Lipase activity, however, remained below 4 U ml⁻¹ for both sardine and tuna by-product-based media. Fish by-products could be used for the production of highly valuable enzymes. The use of fish by-products in producing S. xylosus-growth media can reduce environmental problems associated with waste disposal and, simultaneously, lower the cost of biomass and enzyme production.