Use of the modified Robbins device to study the in vitro biofilm removal efficacy of NitrAdine[trade mark sign], a novel disinfecting formula for the maintenance of oral medical devices

• Published in: Journal of applied microbiology Vol. 105; no. 3; pp. 733 - 740
• Main Authors: Coenye, T, De Prijck, K, De Wever, B, Nelis, H.J
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.09.2008; Blackwell Publishing Ltd; Blackwell Science
• Subjects: biofilm; Biofilms - drug effects; Biological and medical sciences; Candida albicans; Candida albicans - drug effects; Denture Cleansers - pharmacology; Disinfectants - pharmacology; disinfection; Disinfection - methods; Fundamental and applied biological sciences. Psychology; Humans; Microbial Sensitivity Tests - methods; Microbiology; modified Robbins device; Oral Hygiene; oral microbiology; Plankton - drug effects; Staphylococcus aureus; Fungi; Candida albicans; Yeast; Efficiency; Applied microbiology; modified Robbins device; Device; Biofilm; oral microbiology; Disinfection; Fungi Imperfecti; In vitro
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/j.1365-2672.2008.03784.x

To evaluate the use of the modified Robbins device (MRD) to test disinfection strategies against biofilms that form on oral medical devices and to test the biofilm removal efficacy of NitrAdineTM, a disinfectant for the maintenance of oral medical devices. Biofilms were grown on discs using the MRD and biofilms formed in this system were used to evaluate the efficacy of NitrAdineTM and to determine the optimal disinfection conditions. Our data indicate that the use of the MRD allows for the rapid and reproducible formation of high-density biofilms. Determination of the efficacy of NitrAdineTM revealed high activity against biofilms tested (e.g. >3 log reduction for Candida albicans and Staphylococcus aureus) and allowed the determination of the optimal conditions for its use. The high reproducibility and flexibility of the MRD make it an excellent candidate for standardized testing of disinfectants aimed at reducing biofilms on oral medical devices. Using this system, we were able to demonstrate that NitrAdineTM exhibits high activity against biofilms formed by the micro-organisms tested. Our data suggest that our procedure is appropriate for standardized testing of disinfectants aimed at reducing biofilms on oral medical devices.