Monitoring of human herpesviruses‐6 and ‐7 DNA in saliva samples during the acute and convalescent phases of exanthem subitum

• Published in: Journal of medical virology Vol. 89; no. 4; pp. 696 - 702
• Main Authors: Miyazaki, Yuki, Namba, Hikaru, Torigoe, Sadayoshi, Watanabe, Masahiro, Yamashita, Nobuko, Ogawa, Hirohito, Morishima, Tsuneo, Yamada, Masao
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.04.2017
• Subjects: Adult; beta‐herpesviruses; Body fluids; Child; Child, Preschool; Deoxyribonucleic acid; DNA; DNA, Viral - analysis; Exanthema Subitum - virology; Female; Herpes viruses; Herpesviridae; Herpesvirus 6, Human - genetics; Herpesvirus 7, Human - genetics; Humans; Infant; Male; Pediatrics; real‐time PCR; roseola infantum; Saliva - virology; source of infection; Time Factors; Virology; virus isolation; Virus Shedding; virus isolation; roseola infantum; beta-herpesviruses; real-time PCR; virus shedding; source of infection
• ISSN: 0146-6615; 1096-9071; 1096-9071
• DOI: 10.1002/jmv.24690

The amounts of the DNAs of human herpesviruses‐6 (HHV‐6) and ‐7 (HHV‐7) in saliva samples were monitored during the acute and convalescent phases of exanthem subitum (ES) to elucidate the kinetics of virus shedding after ES. A total of 247 saliva samples were collected from 17 children (5 males and 12 females: 8–31 months old at onset). The monitoring period ranged from 152 to 721 days after onset, and in 15 children it was longer than 1 year. Among the 17 cases, 16 were attributed to HHV‐6B, while a single case was attributed to HHV‐7. Detection rates and average amounts of HHV‐6 DNA in saliva samples after ES attributed to HHV‐6B were low in the acute phase, increased to the maximum in the convalescent phase at 3–7 months, and then decreased. In addition, to investigate the source of infection, saliva samples from the older siblings (age 3–9 years) and parents of ES patients and children with a history of ES were also examined. The detection rate of HHV‐6 DNA in saliva samples from 3‐ to 9‐year‐old children was significantly higher than the rate in adult saliva samples. Taken together, these findings suggest that the saliva of children in the convalescent phase of ES might be a more likely source of HHV‐6 infection than that of adults. J. Med. Virol. 89:696–702, 2017. © 2016 Wiley Periodicals, Inc.