Generation of Vascularized Neural Organoids by Co-culturing with Mesodermal Progenitor Cells

• Published in: STAR protocols Vol. 1; no. 1; p. 100041
• Main Authors: Wörsdörfer, Philipp, Rockel, Anna, Alt, Yvonne, Kern, Anna, Ergün, Süleyman
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 19.06.2020; Elsevier
• Subjects: Animals; Cell Culture Techniques, Three Dimensional - methods; Cells, Cultured; Coculture Techniques - methods; Humans; Induced Pluripotent Stem Cells - cytology; Mesoderm - cytology; Mice; Nerve Tissue - blood supply; Nerve Tissue - cytology; Organoids - blood supply; Organoids - cytology; Protocol
• ISSN: 2666-1667; 2666-1667
• DOI: 10.1016/j.xpro.2020.100041

Organoids are three-dimensional (3D) constructs generated in stem cell cultures and are thought to mimic tissue and organ development in situ. However, until recently, they often exclusively recapitulated the development of the organ`s parenchyma without the major components of the organ stroma. Here, we describe a protocol to incorporate stromal components, first of all blood vessels, by co-culturing with induced pluripotent stem cell-derived mesodermal progenitor cells. For complete details on the use and execution of this protocol, please refer to Wörsdörfer et al. (2019). [Display omitted] •Generation of vascularized neural organoids•Analysis of tissue morphogenesis•3D reconstruction of vascular networks Organoids are three-dimensional (3D) constructs generated in stem cell cultures and are thought to mimic tissue and organ development in situ. However, until recently, they often exclusively recapitulated the development of the organ`s parenchyma without the major components of the organ stroma. Here, we describe a protocol to incorporate stromal components, first of all blood vessels, by co-culturing with induced pluripotent stem cell-derived mesodermal progenitor cells.