Generation of human iPSCs from urine derived cells of a patient with a novel homozygous PAI-1 mutation

• Published in: Stem cell research Vol. 17; no. 3; pp. 657 - 660
• Main Authors: Afzal, Muhammad Zeeshan, Gartz, Melanie, Klyachko, Ekaterina A., Khan, Sadiya Sana, Shah, Sanjiv J., Gupta, Sweta, Shapiro, Amy D., Vaughan, Douglas E., Strande, Jennifer L.
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 01.11.2016
• Subjects: Base Sequence; Cell Differentiation; Cell Line; Cellular Reprogramming; DNA Mutational Analysis; Embryoid Bodies - cytology; Embryoid Bodies - metabolism; Female; Genetic Vectors - genetics; Genetic Vectors - metabolism; Homozygote; Humans; Induced Pluripotent Stem Cells - cytology; Induced Pluripotent Stem Cells - metabolism; Karyotype; Microscopy, Fluorescence; Mutagenesis, Insertional; Plasminogen Activator Inhibitor 1 - genetics; Sendai virus - genetics; Transcription Factors - genetics; Transcription Factors - metabolism; Urine - cytology

We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from subject with a novel homozygous Plasminogen Activator Inhibitor-1 (PAI-1 null) mutation. The Sendai virus (SeV) vector encoding pluripotent Yama...