Generation of human iPSCs from urine derived cells of a patient with a novel homozygous PAI-1 mutation

We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from subject with a novel homozygous Plasminogen Activator Inhibitor-1 (PAI-1 null) mutation. The Sendai virus (SeV) vector encoding pluripotent Yama...

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Bibliographic Details
Published inStem cell research Vol. 17; no. 3; pp. 657 - 660
Main Authors Afzal, Muhammad Zeeshan, Gartz, Melanie, Klyachko, Ekaterina A., Khan, Sadiya Sana, Shah, Sanjiv J., Gupta, Sweta, Shapiro, Amy D., Vaughan, Douglas E., Strande, Jennifer L.
Format Journal Article
LanguageEnglish
Published England Elsevier B.V 01.11.2016
Subjects
Base Sequence
Cell Differentiation
Cell Line
Cellular Reprogramming
DNA Mutational Analysis
Embryoid Bodies - cytology
Embryoid Bodies - metabolism
Female
Genetic Vectors - genetics
Genetic Vectors - metabolism
Homozygote
Humans
Induced Pluripotent Stem Cells - cytology
Induced Pluripotent Stem Cells - metabolism
Karyotype
Microscopy, Fluorescence
Mutagenesis, Insertional
Plasminogen Activator Inhibitor 1 - genetics
Sendai virus - genetics
Transcription Factors - genetics
Transcription Factors - metabolism
Urine - cytology
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Summary:We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from subject with a novel homozygous Plasminogen Activator Inhibitor-1 (PAI-1 null) mutation. The Sendai virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at a low multiplicity of infection to reprogram the PAI-1 UCs.
ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2016.11.010