Generation of human iPSCs from urine derived cells of a patient with a novel homozygous PAI-1 mutation

We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from subject with a novel homozygous Plasminogen Activator Inhibitor-1 (PAI-1 null) mutation. The Sendai virus (SeV) vector encoding pluripotent Yama...

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Published inStem cell research Vol. 17; no. 3; pp. 657 - 660
Main Authors Afzal, Muhammad Zeeshan, Gartz, Melanie, Klyachko, Ekaterina A., Khan, Sadiya Sana, Shah, Sanjiv J., Gupta, Sweta, Shapiro, Amy D., Vaughan, Douglas E., Strande, Jennifer L.
Format Journal Article
LanguageEnglish
Published England Elsevier B.V 01.11.2016
Subjects
Base Sequence
Cell Differentiation
Cell Line
Cellular Reprogramming
DNA Mutational Analysis
Embryoid Bodies - cytology
Embryoid Bodies - metabolism
Female
Genetic Vectors - genetics
Genetic Vectors - metabolism
Homozygote
Humans
Induced Pluripotent Stem Cells - cytology
Induced Pluripotent Stem Cells - metabolism
Karyotype
Microscopy, Fluorescence
Mutagenesis, Insertional
Plasminogen Activator Inhibitor 1 - genetics
Sendai virus - genetics
Transcription Factors - genetics
Transcription Factors - metabolism
Urine - cytology
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Abstract We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from subject with a novel homozygous Plasminogen Activator Inhibitor-1 (PAI-1 null) mutation. The Sendai virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at a low multiplicity of infection to reprogram the PAI-1 UCs.
AbstractList We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from subject with a novel homozygous Plasminogen Activator Inhibitor-1 (PAI-1 null) mutation. The Sendai virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at a low multiplicity of infection to reprogram the PAI-1 UCs.
We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from subjects with a novel homozygous Plasminogen Activator Inhibitor-1 (PAI-1 null) mutation. The Sendai virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at a low multiplicity of infection to reprogram the PAI-1 UCs.
Author Afzal, Muhammad Zeeshan
Klyachko, Ekaterina A.
Shah, Sanjiv J.
Shapiro, Amy D.
Strande, Jennifer L.
Khan, Sadiya Sana
Vaughan, Douglas E.
Gartz, Melanie
Gupta, Sweta
AuthorAffiliation a Department of Medicine and Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI
c Indiana Hemophilia and Thrombosis Center, Indianapolis, IN
b Department of Medicine, Northwestern University-Feinberg School of Medicine, Chicago, IL
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  organization: Department of Medicine and Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI, United States
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Cites_doi 10.1055/s-0034-1387883
10.1160/TH14-08-0678
10.1111/acel.12247
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10.1160/TH05-07-0492
10.1055/s-0034-1384635
10.3791/52032
10.1016/j.cell.2007.11.019
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Snippet We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from...
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StartPage 657
SubjectTerms Base Sequence
Cell Differentiation
Cell Line
Cellular Reprogramming
DNA Mutational Analysis
Embryoid Bodies - cytology
Embryoid Bodies - metabolism
Female
Genetic Vectors - genetics
Genetic Vectors - metabolism
Homozygote
Humans
Induced Pluripotent Stem Cells - cytology
Induced Pluripotent Stem Cells - metabolism
Karyotype
Microscopy, Fluorescence
Mutagenesis, Insertional
Plasminogen Activator Inhibitor 1 - genetics
Sendai virus - genetics
Transcription Factors - genetics
Transcription Factors - metabolism
Urine - cytology
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Title Generation of human iPSCs from urine derived cells of a patient with a novel homozygous PAI-1 mutation
URI https://dx.doi.org/10.1016/j.scr.2016.11.010
https://www.ncbi.nlm.nih.gov/pubmed/27934602
https://pubmed.ncbi.nlm.nih.gov/PMC5409870
Volume 17
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