Environmental Microcystin exposure in underlying NAFLD-induced exacerbation of neuroinflammation, blood-brain barrier dysfunction, and neurodegeneration are NLRP3 and S100B dependent

• Published in: Toxicology (Amsterdam) Vol. 461; p. 152901
• Main Authors: Mondal, Ayan, Saha, Punnag, Bose, Dipro, Chatterjee, Somdatta, Seth, Ratanesh K., Xiao, Shuo, Porter, Dwayne E., Brooks, Bryan W., Scott, Geoff I., Nagarkatti, Mitzi, Nagarkatti, Prakash, Chatterjee, Saurabh
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier B.V 01.09.2021
• Subjects: Animals; Apoptosis - drug effects; Blood-brain barrier; Blood-Brain Barrier - drug effects; Blood-Brain Barrier - pathology; Disease Models, Animal; Environmental Exposure - adverse effects; Inflammasomes - metabolism; Inflammation - pathology; Lipocalin2; Male; Marine Toxins - toxicity; Mice; Mice, Inbred C57BL; Mice, Knockout; Microcystin-LR; Microcystins - toxicity; NAFLD; NASH; Neuroinflammatory Diseases - physiopathology; NLR Family, Pyrin Domain-Containing 3 Protein - genetics; Non-alcoholic Fatty Liver Disease - physiopathology; Oxidative Stress - drug effects; S100 Calcium Binding Protein beta Subunit - metabolism; S100B; NAFLD; Lipocalin2; Blood-brain barrier; NASH; S100B; Microcystin-LR
• ISSN: 0300-483X; 1879-3185
• DOI: 10.1016/j.tox.2021.152901

Nonalcoholic fatty liver disease (NAFLD) has been shown to be associated with extrahepatic comorbidities including neuronal inflammation and Alzheimer’s-like pathology. Environmental and genetic factors also act as a second hit to modulate severity and are expected to enhance the NAFLD-linked neuropathology. We hypothezied that environmental microcystin-LR (MC-LR), a toxin produced by harmful algal blooms of cyanobacteria, exacerbates the neuroinflammation and degeneration of neurons associated with NAFLD. Using a mouse model of NAFLD, exposed to MC-LR subsequent to the onset of fatty liver, we show that the cyanotoxin could significantly increase proinflammatory cytokine expression in the frontal cortex and cause increased expression of Lcn2 and HMGB1. The above effects were NLRP3 inflammasome activation-dependent since the use of NLRP3 knockout mice abrogated the increase in inflammation. NLRP3 was also responsible for decreased expression of the blood-brain barrier (BBB) tight junction proteins Occludin and Claudin 5 suggesting BBB dysfunction was parallel to neuroinflammation following microcystin exposure. An increased circulatory S100B release, a hallmark of astrocyte activation in MC-LR exposed NAFLD mice also confirmed BBB integrity loss, but the astrocyte activation observed in vivo was NLRP3 independent suggesting an important role of a secondary S100B mediated crosstalk. Mechanistically, conditioned medium from reactive astrocytes and parallel S100B incubation in neuronal cells caused increased inducible NOS, COX-2, and higher BAX/ Bcl2 protein expression suggesting oxidative stress-mediated neuronal cell apoptosis crucial for neurodegeneration. Taken together, MC-LR exacerbated neuronal NAFLD-linked comorbidities leading to cortical inflammation, BBB dysfunction, and neuronal apoptosis.