Participation of nitric oxide reductase in survival of Pseudomonas aeruginosa in LPS-activated macrophages

• Published in: Biochemical and biophysical research communications Vol. 355; no. 2; pp. 587 - 591
• Main Authors: Kakishima, Kohei, Shiratsuchi, Akiko, Taoka, Azuma, Nakanishi, Yoshinobu, Fukumori, Yoshihiro
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 06.04.2007
• Subjects: Animals; Base Sequence; Cell Line; Denitrification; DNA Primers; Intracellular survival; Lipopolysaccharides - pharmacology; Macrophage Activation - drug effects; Macrophages - drug effects; Macrophages - enzymology; Mice; Nitric Oxide - pharmacology; Nitric oxide reductase; Nitrosative stress; Oxidoreductases - metabolism; Pseudomonas aeruginosa; Pseudomonas aeruginosa - drug effects; Pseudomonas aeruginosa - enzymology; Pseudomonas aeruginosa - growth & development; Denitrification; Pseudomonas aeruginosa; Intracellular survival; Nitric oxide reductase; Nitrosative stress
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2007.02.017

Nitric oxide (NO) plays a crucial role in the antimicrobial activity of host defense systems. We investigated the function of Pseudomonas aeruginosa NO reductase as a detoxifying enzyme in phagocytes. We found that the growth of the NO reductase-deficient mutant of P. aeruginosa under a microaerobic condition was inhibited by the exogenous NO. Furthermore, the intracellular survival assay within the NO-producing RAW 264.7 macrophages revealed that the wild-type strain survived longer than the NO reductase-deficient mutant. These results suggest that the P. aeruginosa NO reductase may contribute to the intracellular survival by acting as a counter component against the host’s defense systems.