Halobacterium piscisalsi sp. nov., from fermented fish (pla-ra) in Thailand

• Published in: International journal of systematic and evolutionary microbiology Vol. 58; no. 9; pp. 2136 - 2140
• Main Authors: Yachai, Mongkol, Tanasupawat, Somboon, Itoh, Takashi, Benjakul, Soottawat, Visessanguan, Wonnop, Valyasevi, Ruud
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.09.2008; Society for General Microbiology
• Subjects: Bacteriology; Base Composition; Biological and medical sciences; Carbohydrate Metabolism; DNA, Archaeal - chemistry; DNA, Archaeal - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Fish Products - microbiology; Fundamental and applied biological sciences. Psychology; Genes, rRNA; Halobacteriaceae; Halobacterium; Halobacterium - classification; Halobacterium - genetics; Halobacterium - isolation & purification; Halobacterium - physiology; Halobacterium piscisalsi; Halobacterium salinarum; Hydrogen-Ion Concentration; Hypotonic Solutions - toxicity; Locomotion; Microbial Viability; Microbiology; Molecular Sequence Data; Nucleic Acid Hybridization; Phospholipids - analysis; Phylogeny; RNA, Archaeal - genetics; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Sequence Homology, Nucleic Acid; Sodium Chloride - metabolism; Systematics; Temperature; Thailand; Thailand; Asia; Edible fish; Archaeobacteria; Taxonomy; Halobacteriales; Bacteria; Halobacterium; New species; Halobacteriaceae; Fermented product; Traditional food stuff
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.65592-0

1 Department of Food Technology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand 2 Department of Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand 3 Japan Collection of Microorganisms, RIKEN BioResource Center, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan 4 National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Klong 1, Klong Luang, Pathumthani 12120, Thailand Correspondence Wonnop Visessanguan wonnop{at}biotec.or.th A Gram-negative, motile, rod-shaped, extremely halophilic archaeon, designated strain HPC1-2 T , was isolated from pla-ra , a salt-fermented fish product of Thailand. Strain HPC1-2 T was able to grow at 20–60 °C (optimum at 37–40 °C), at 2.6–5.1 M NaCl (optimum at 3.4–4.3 M NaCl) and at pH 5.0–8.0 (optimum at pH 7.0–7.5). Hypotonic treatment with less than 1.7 M NaCl caused cell lysis. The major polar lipids of the isolate were C 20 C 20 derivatives of phosphatidylglycerol, methylated phosphatidylglycerol phosphate, phosphatidylglycerol sulfate, triglycosyl diether, sulfated triglycosyl diether and sulfated tetraglycosyl diether. The G+C content of the DNA was 65.5 mol%. 16S rRNA gene sequence analysis indicated that the isolate represented a member of the genus Halobacterium in the family Halobacteriaceae . Based on 16S rRNA gene sequence similarity, strain HPC1-2 T was related most closely to Halobacterium salinarum DSM 3754 T (99.2 %) and Halobacterium jilantaiense JCM 13558 T (97.8 %). However, low levels of DNA–DNA relatedness suggested that strain HPC1-2 T was genotypically different from these closely related type strains. Strain HPC1-2 T could also be differentiated based on physiological and biochemical characteristics. Therefore, strain HPC1-2 T is considered to represent a novel species of the genus Halobacterium , for which the name Halobacterium piscisalsi sp. nov. is proposed. The type strain is HPC1-2 T (=BCC 24372 T =JCM 14661 T =PCU 302 T ). Abbreviations: PG, phosphatidylglycerol; PGP-Me, methylated phosphatidylglycerol phosphate; PGS, phosphatidylglycerol sulfate; S-TeGD, sulfated tetraglycosyl diether; S-TGD, sulfated triglycosyl diether; TGD, triglycosyl diether The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain HPC1-2 T is AB285020 . Maximum-parsimony and maximum-likelihood phylogenetic trees showing the relationships between strain HPC1-2 T and related archaeal species based on 16S rRNA gene sequences are available with the online version of this paper.