Reclassification of Nocardia corynebacterioides Serrano et al. 1972 (Approved Lists 1980) as Rhodococcus corynebacterioides comb. nov

• Published in: International journal of systematic and evolutionary microbiology Vol. 55; no. 3; pp. 1345 - 1348
• Main Authors: Yassin, A. F, Schaal, K. P
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.05.2005; Society for General Microbiology
• Subjects: Bacterial Typing Techniques; Bacteriology; Biological and medical sciences; Corynebacterium; Dietzia; DNA, Bacterial - chemistry; DNA, Bacterial - isolation & purification; DNA, Ribosomal - chemistry; DNA, Ribosomal - isolation & purification; Fundamental and applied biological sciences. Psychology; Genes, rRNA; Gordonia; Microbiology; Miscellaneous; Molecular Sequence Data; Mycobacterium; Nocardia - classification; Nocardia - cytology; Nocardia - genetics; Nocardia - physiology; Nocardia corynebacterioides; Phylogeny; Rhodococcus; Rhodococcus - classification; Rhodococcus - cytology; Rhodococcus - genetics; Rhodococcus - physiology; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Systematics; Vitamin K 2 - analysis; Vitamin K 2 - isolation & purification; Actinomycetales; Taxonomic review; Gordona; Nocardia; Taxonomy; Bacteria; Nocardiaceae; Rhodococcus; Actinomycetes
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.63529-0

Institut für Medizinische Mikrobiologie und Immunologie der Universität Bonn, 53127 Bonn, Germany Correspondence A. F. Yassin yassin{at}mibi03.meb.uni-bonn.de The type strain of Nocardia corynebacterioides was the subject of a polyphasic taxonomic study. The 16S rRNA gene sequence was aligned with the sequences of representatives of the genera Corynebacterium , Dietzia , Gordonia , Mycobacterium , Nocardia , Rhodococcus , Skermania , Tsukamurella and Williamsia , and phylogenetic trees were constructed by using maximum-parsimony, maximum-likelihood and neighbour-joining methods. It was evident from the phylogenetic analysis that N. corynebacterioides represents a distinct phyletic line within the genus Rhodococcus . Menaquinone analysis showed that the organism contained dihydrogenated menaquinone with eight isoprene units, MK-8(H 2 ), as the major isoprenologue. The genealogical evidence, together with chemotaxonomic and phenotypic data from this and previous studies, indicates that N. corynebacterioides DSM 20151 T (=CIP 104510 T ) should be reclassified in the genus Rhodococcus as Rhodococcus corynebacterioides comb. nov. Published online ahead of print on 21 January 2005 as DOI 10.1099/ijs.0.63529-0. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of Rhodococcus corynebacterioides DSM 20151 T is X80615 .