Antimicrobial Activity and Mechanism of Essential Oil of Endemic Salvia hypargeia Finc. & Mey. in Turkey

• Published in: Indian journal of microbiology Vol. 61; no. 3; pp. 291 - 297
• Main Author: Erdoğan Eliuz, Elif Ayşe
• Format: Journal Article
• Language: English
• Published: New Delhi Springer India 01.09.2021; Springer Nature B.V
• Subjects: Antiinfectives and antibacterials; Antimicrobial activity; Antimicrobial agents; Biomedical and Life Sciences; Camphor; Cineole; Deoxyribonucleic acid; DNA; Essential oils; Life Sciences; Medical Microbiology; Microbiology; Microorganisms; Oils & fats; Original; Original Research Article; Potassium; Proteins; Terpineol; α-Pinene; Antimicrobial; Acinetobacter baumannii
• ISSN: 0046-8991; 0973-7715
• DOI: 10.1007/s12088-021-00939-1

In this study, the aerial parts of Salvia hypargeia were subjected to hydrodistillation and the resulting compounds were analyzed in GC–MS. Antimicrobial activity of S. hypargeia essential oil (EO) against A. baumannii , S. aureus , and C. tropicalis were determined by agar well diffusion assay and microdilution method. Antimicrobial mechanism of the EO were investigated based on change of TTC-dehydrogenase relative, leakages of intracellular protein, DNA and potassium ion (K +). The main components of the EO were β-pinene, 1,8-cineole, camphor, α-pinene, 4-terpineol, and 4-thujanol. The MICs of the EO against the microorganisms were 15.2 mg/mL for S. aureus, 17.5 mg/mL for C. tropicalis and 28.8 mg/mL for A. baumannii. The inhibition zones were 18.16 mm, 25.01 mm, and 27.01 mm for A. baumannii , S. aureus , and C. tropicalis, respectively ( p  < 0.05). An increase in DNA, protein and K + leakage was observed when microorganisms were exposed to the EO. The TTC-DRA of the treated microorganism cells was also significantly decreased because of slowing the respiration. The present study provided an experimental basis of practical application of S. hypargeia EO as a natural agent.