Identification MdeSOS1 in Magnolia denudata and its function in response to salt stress

• Published in: Journal of plant interactions Vol. 15; no. 1; pp. 417 - 426
• Main Authors: Chang, Pengjie, Wu, Zhen, Song, Nannan, Bian, Sainan, Wang, Ninghang, Xuan, Lingjuan, Dong, Bin, Wang, Xiaode, Shen, Yamei
• Format: Journal Article
• Language: English
• Published: Philadelphia Taylor & Francis 01.01.2020; Taylor & Francis Ltd; Taylor & Francis Group
• Subjects: Abiotic stress; antiporter; Fusion protein; Homology; Magnolia denudata; Membranes; na+/h+ antiporter; Na+/H+-exchanging ATPase; Open reading frames; Proteins; Salinity tolerance; salt stress; Salt tolerance; Seedlings; Sodium chloride; SOS1; transgenic plants
• ISSN: 1742-9145; 1742-9153
• DOI: 10.1080/17429145.2020.1843721

In this study, annual Magnolia denudata seedlings were treated with a 200 mmol L −1 NaCl solution. Na + content in its stems increased by more than about 24 times after 72 h and K + content in leaves basically maintained in a steady state. MdeSOS1, isolated from M. denudata, comprised a 3453-bp ORF and included 12 transmembrane structures within its N terminal and a hydrophilic tail in its C-terminal. Its protein shared the identity of 68.3% and 62.7% at the peptide level with the homologue PeSOS1 and AtSOS1 respectively. The MdeSOS1 was significantly induced to up-regulation in various tissues exposed to salt stress and improved the salt tolerance of Arabidopsis. Our results also revealed that the MdeSOS1-GFP fusion protein was located on the plasma membrane and MdeSOS1 encoded a salt-inducible plasma membrane Na + /H + antiporter, which provides a reference to improve the salt tolerance of Magnolia species by transgenic approaches.