Generation of human iPSCs from urine derived cells of a non-affected control subject

We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from non-affected control subjects to use as a comparison group for the iPSC lines containing a Plasminogen Activator Inhibitor-1 (PAI-1 homozygous/h...

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Published inStem cell research Vol. 18; pp. 33 - 36
Main Authors Afzal, Muhammad Zeeshan, Gartz, Melanie, Klyachko, Ekaterina A., Khan, Sadiya Sana, Shah, Sanjiv J., Gupta, Sweta, Shapiro, Amy D., Vaughan, Douglas E., Strande, Jennifer L.
Format Journal Article
LanguageEnglish
Published England Elsevier B.V 01.01.2017
Subjects
Base Sequence
Cell Culture Techniques - methods
Cell Line
Cellular Reprogramming
Embryoid Bodies - metabolism
Embryoid Bodies - pathology
Female
Genetic Vectors - genetics
Genetic Vectors - metabolism
Genotype
Heterozygote
Homozygote
Humans
Induced Pluripotent Stem Cells - cytology
Induced Pluripotent Stem Cells - metabolism
Karyotype
Microscopy, Fluorescence
Plasminogen Activator Inhibitor 1 - genetics
Plasminogen Activator Inhibitor 1 - metabolism
Sendai virus - genetics
Sequence Analysis, DNA
Transcription Factors - genetics
Transcription Factors - metabolism
Urine - cytology
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Abstract We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from non-affected control subjects to use as a comparison group for the iPSC lines containing a Plasminogen Activator Inhibitor-1 (PAI-1 homozygous/heterozygous) mutation. The Sendai Virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at a low multiplicity of infection to reprogram the UCs.
AbstractList We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from non-affected control subjects to use as a comparison group for the iPSC lines containing a Plasminogen Activator Inhibitor-1 (PAI-1 null) mutation. The Sendai virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at a low multiplicity of infection to reprogram the UCs.
We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from non-affected control subjects to use as a comparison group for the iPSC lines containing a Plasminogen Activator Inhibitor-1 (PAI-1 homozygous/heterozygous) mutation. The Sendai Virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at a low multiplicity of infection to reprogram the UCs.
We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from non-affected control subjects to use as a comparison group for the iPSC lines containing a Plasminogen Activator Inhibitor-1 (PAI-1 homozygous/heterozygous) mutation. The Sendai Virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at a low multiplicity of infection to reprogram the UCs.We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from non-affected control subjects to use as a comparison group for the iPSC lines containing a Plasminogen Activator Inhibitor-1 (PAI-1 homozygous/heterozygous) mutation. The Sendai Virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at a low multiplicity of infection to reprogram the UCs.
Author Afzal, Muhammad Zeeshan
Klyachko, Ekaterina A.
Shah, Sanjiv J.
Shapiro, Amy D.
Strande, Jennifer L.
Khan, Sadiya Sana
Vaughan, Douglas E.
Gartz, Melanie
Gupta, Sweta
AuthorAffiliation a Department of Medicine and Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI
c Indiana Hemophilia and Thrombosis Center, Indianapolis, IN
b Department of Medicine, Northwestern University-Feinberg School of Medicine, Chicago, IL
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Cites_doi 10.1016/j.cell.2007.11.019
10.1056/NEJM199212103272406
10.1182/blood.V90.1.204
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9207454 - Blood. 1997 Jul 1;90(1):204-8
18035408 - Cell. 2007 Nov 30;131(5):861-72
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– reference: 9207454 - Blood. 1997 Jul 1;90(1):204-8
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SubjectTerms Base Sequence
Cell Culture Techniques - methods
Cell Line
Cellular Reprogramming
Embryoid Bodies - metabolism
Embryoid Bodies - pathology
Female
Genetic Vectors - genetics
Genetic Vectors - metabolism
Genotype
Heterozygote
Homozygote
Humans
Induced Pluripotent Stem Cells - cytology
Induced Pluripotent Stem Cells - metabolism
Karyotype
Microscopy, Fluorescence
Plasminogen Activator Inhibitor 1 - genetics
Plasminogen Activator Inhibitor 1 - metabolism
Sendai virus - genetics
Sequence Analysis, DNA
Transcription Factors - genetics
Transcription Factors - metabolism
Urine - cytology
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Title Generation of human iPSCs from urine derived cells of a non-affected control subject
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