Generation of human iPSCs from urine derived cells of a non-affected control subject

• Published in: Stem cell research Vol. 18; pp. 33 - 36
• Main Authors: Afzal, Muhammad Zeeshan, Gartz, Melanie, Klyachko, Ekaterina A., Khan, Sadiya Sana, Shah, Sanjiv J., Gupta, Sweta, Shapiro, Amy D., Vaughan, Douglas E., Strande, Jennifer L.
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 01.01.2017
• Subjects: Base Sequence; Cell Culture Techniques - methods; Cell Line; Cellular Reprogramming; Embryoid Bodies - metabolism; Embryoid Bodies - pathology; Female; Genetic Vectors - genetics; Genetic Vectors - metabolism; Genotype; Heterozygote; Homozygote; Humans; Induced Pluripotent Stem Cells - cytology; Induced Pluripotent Stem Cells - metabolism; Karyotype; Microscopy, Fluorescence; Plasminogen Activator Inhibitor 1 - genetics; Plasminogen Activator Inhibitor 1 - metabolism; Sendai virus - genetics; Sequence Analysis, DNA; Transcription Factors - genetics; Transcription Factors - metabolism; Urine - cytology
• ISSN: 1873-5061; 1876-7753; 1876-7753
• DOI: 10.1016/j.scr.2016.12.008

We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from non-affected control subjects to use as a comparison group for the iPSC lines containing a Plasminogen Activator Inhibitor-1 (PAI-1 homozygous/heterozygous) mutation. The Sendai Virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at a low multiplicity of infection to reprogram the UCs.