Binding of retinoids and β-carotene to β-lactoglobulin. Influence of protein modifications

• Published in: Biochimica et biophysica acta Vol. 1079; no. 3; pp. 316 - 320
• Main Authors: Dufour, Eric, Haertlé, Tomasz
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 20.09.1991; Elsevier
• Subjects: Analytical, structural and metabolic biochemistry; beta -carotene; Binding and carrier proteins; Biological and medical sciences; Carotenoids - metabolism; Fundamental and applied biological sciences. Psychology; Kinetics; Lactoglobulins - metabolism; Life Sciences; Ligand binding; Modification; Protein Binding; Proteins; retinoic acid; Retinoid; Retinoids - metabolism; retinol; retinyl acetate; Spectrometry, Fluorescence; Tretinoin - metabolism; Vitamin A - analogs & derivatives; Vitamin A - metabolism; ß-Carotene; ß-Lactoglobulin; Modification; Ligand binding; N-ethyllysyl-BLG; ß-Carotene; Retinoid; ß-Lactoglobulin; MetBLG; BLG; Lactoglobulins; Carotene; Dissociation constant; Retinoids; Carrier protein; Fluorescence spectrometry

The binding of retinol, retinyl acetate, retinoic acid and ß-carotene to native, esterified and alkylated ß-lactoglobulin was followed by quenching of tryptophan fluorescence. Three studied retinoids bind to native or modified ß-lactoglobulin in 1 : 1 molar ratios, with apparent dissociation constan...