Evaluation of the extraction and stability of chlorophyll-rich extracts by supercritical fluid chromatography

In this study, a rapid (less than 10 min) analytical method by reverse-phase supercritical fluid chromatography was developed with an isocratic mobile phase, enabling the separation of 11 compounds, chlorophyll a and b , pheophytin a and numerous allomers or epimers. This method was used to examine...

Full description

Saved in:
Bibliographic Details
Published inAnalytical and bioanalytical chemistry Vol. 412; no. 26; pp. 7263 - 7273
Main Authors Lefebvre, Thibault, Destandau, Emilie, Lesellier, Eric
Format Journal Article
LanguageEnglish
Published Berlin/Heidelberg Springer Berlin Heidelberg 01.10.2020
Springer
Springer Nature B.V
Subjects
Analysis
Analytical Chemistry
Biochemistry
Biological products
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Chlorophyll
Chlorophyll - chemistry
Chlorophyll - isolation & purification
Chromatography
Chromatography, Supercritical Fluid - methods
Ethanol
Evaporation
Food Science
Laboratory Medicine
Monitoring/Environmental Analysis
Phaeophytin
Pigments
Plant extracts
Plant Extracts - chemistry
Research Paper
Spectrophotometry, Ultraviolet
Stability analysis
Supercritical fluids
Ultrasound
United Arab Emirates
Chlorophyll
Plant extraction
Sample stability
Supercritical fluid chromatography
Online AccessGet full text

Cover

More Information
Summary:In this study, a rapid (less than 10 min) analytical method by reverse-phase supercritical fluid chromatography was developed with an isocratic mobile phase, enabling the separation of 11 compounds, chlorophyll a and b , pheophytin a and numerous allomers or epimers. This method was used to examine the stability of chlorophyll pigments of plant extracts obtained with various extraction methods including microwave-assisted extraction (MAE), supercritical fluid extraction (SFE), pressurized liquid extraction (PLE) and ultrasound-assisted extraction (UAE), with ethanol as solvent or modifier. The effect of storage was studied for both liquid and dried extracts. Irrespective of the extraction method, the evaporation of the extracts induced partial degradation of the chlorophyll pigments. It was found that liquid extracts could be stored at 4 °C for 3 weeks without a dramatic change in allomer forms of chlorophylls. However, during this storage period, epimerization appears to be important, leading to a significant decrease in the chlorophyll b native form. Graphical abstract
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-020-02859-3