Identification of the suppressive factors for human immunodeficiency virus type-1 replication using the siRNA mini-library directed against host cellular genes

• Published in: Biochemical and biophysical research communications Vol. 359; no. 3; pp. 729 - 734
• Main Authors: Kameoka, Masanori, Kitagawa, Yukiko, Utachee, Piraporn, Jinnopat, Piyamat, Dhepakson, Panadda, Isarangkura-na-ayuthaya, Panasda, Tokunaga, Kenzo, Sato, Hironori, Komano, Jun, Yamamoto, Naoki, Oguchi, Shinobu, Natori, Yukikazu, Ikuta, Kazuyoshi
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 03.08.2007
• Subjects: Cell Line; Gene Expression Regulation; Gene Library; HIV-1 - physiology; Host factor; Human immunodeficiency virus 1; Human immunodeficiency virus type-1; Humans; RNA interference; RNA, Small Interfering - genetics; siRNA mini-library; Small interfering RNA; Suppressor Factors, Immunologic - genetics; Suppressor Factors, Immunologic - metabolism; Virus Replication - physiology; siRNA mini-library; Small interfering RNA; RNA interference; Human immunodeficiency virus type-1; Host factor
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2007.05.173

We performed the screening to find the novel host factors affecting human immunodeficiency virus type-1 (HIV-1) replication using the siRNA mini-library consisted with 257 siRNAs directed against cellular genes. J111 cells, a human acute monocytic leukemia cell line, were transfected with individual siRNA, followed by either infected or transfected with the HIV-1 molecular clone with luciferase reporter gene in 96-well plate format. The results showed that six siRNAs significantly enhanced the HIV-1 replication in J111 cells, indicating that the target cellular genes of those siRNAs may negatively regulate HIV-1 replication in normal cell culture condition. We also discuss the possible mechanisms by which those cellular proteins regulate viral replication.