Generation of human iPSCs from urine derived cells of patient with a novel heterozygous PAI-1 mutation

We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from subjects heterozygous for a novel Plasminogen Activator Inhibitor-1 (PAI-1) mutation. The Sendai Virus (SeV) vector encoding pluripotent Yamanak...

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Published inStem cell research Vol. 18; pp. 41 - 44
Main Authors Afzal, Muhammad Zeeshan, Gartz, Melanie, Klyachko, Ekaterina A., Khan, Sadiya Sana, Shah, Sanjiv J., Gupta, Sweta, Shapiro, Amy D., Vaughan, Douglas E., Strande, Jennifer L.
Format Journal Article
LanguageEnglish
Published England Elsevier B.V 01.01.2017
Subjects
Cell Culture Techniques
Cell Differentiation
Cell Line
Cellular Reprogramming
Embryoid Bodies - metabolism
Embryoid Bodies - pathology
Female
Genetic Vectors - genetics
Genetic Vectors - metabolism
Heterozygote
Humans
Induced Pluripotent Stem Cells - cytology
Induced Pluripotent Stem Cells - metabolism
Karyotype
Microscopy, Fluorescence
Plasminogen Activator Inhibitor 1 - genetics
Polymorphism, Genetic
Sendai virus - genetics
Urine - cytology
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Summary:We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from subjects heterozygous for a novel Plasminogen Activator Inhibitor-1 (PAI-1) mutation. The Sendai Virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at a low multiplicity of infection to reprogram the PAI-1 UCs.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1873-5061
1876-7753
1876-7753
DOI:10.1016/j.scr.2016.12.003