Chemical characterization of fatty acids, alkanes, n-diols and alkyl esters produced by a mixed culture of Trichoderma koningii and Penicillium janthinellum grown aerobically on undecanoic acid, potatoe dextrose and their mixture

• Published in: Journal of environmental science and health. Part B, Pesticides, food contaminants, and agricultural wastes Vol. 51; no. 5; pp. 326 - 339
• Main Authors: Monreal, Carlos M., Chahal, Amarpreet, Schnitzer, Morris, Rowland, Owen
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 03.05.2016; Taylor & Francis Ltd
• Subjects: Aerobiosis; Agar - chemistry; alcohols; Alkanes - metabolism; alkyl esters; Bacteria; Cell culture; Culture Media - chemistry; Esters - metabolism; Fatty acids; Fatty Acids - metabolism; Lipids; NMR; NMR and Py-FIMS; Nuclear magnetic resonance; Penicillium - metabolism; Penicillium janthinellum; potatoe dextrose broth; Potatoes; Solanum tuberosum; Trichoderma - metabolism; Trichoderma koningii; undecanoic acid; potatoe dextrose broth; Lipids; alcohols; NMR and Py-FIMS; Trichoderma koningii; undecanoic acid; Penicillium janthinellum; alkyl esters
• ISSN: 1532-4109; 0360-1234; 1532-4109
• DOI: 10.1080/03601234.2015.1128746

Little is known about the mixed fungal synthesis of high-value aliphatics derived from the metabolism of simple and complex carbon substrates. Trichoderma koningii and Penicillium janthinellum were fed with undecanoic acid (UDA), potatoe dextrose broth (PDB), and their mixture. Pyrolysis Field Ionization Mass Spectrometry (Py-FIMS) together with ¹H and ¹³C Nuclear Magnetic Resonance (NMR) characterized CHCl ₃ soluble aliphatics in the fungal cell culture. Data from NMR and Py-FIMS analysis were complementary to each other. On average, the mixed fungal species produced mostly fatty acids (28% of total ion intensity, TII) > alkanes (2% of TII) > n-diols (2% of TII) > and alkyl esters (0.8% of TII) when fed with UDA, PDB or UDA+PDB. The cell culture accumulated aliphatics extracellularly, although most of the identified compounds accumulated intracellularly. The mixed fungal culture produced high-value chemicals from the metabolic conversion of simple and complex carbon substrates.