A Mutant of Escherichia coli that Requires High Concentrations of Biotin

A recessive mutation (bir) of E. coli, at 77 min on the standard map, destroys ability of the bacteria to take up biotin or to grow except at high concentrations of biotin. In a biotin prototroph, the bir mutation causes overproduction and excretion of biotin, and derepression of at least one biosyn...

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Bibliographic Details
Published inProceedings of the National Academy of Sciences - PNAS Vol. 69; no. 3; pp. 676 - 680
Main Authors Campbell, Allan, Del Campillo-Campbell, Alice, Chang, Robin
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences of the United States of America 01.03.1972
National Acad Sciences
Subjects
Biological Sciences: Genetics
Biosynthesis
Biotin - biosynthesis
Biotin - metabolism
Caproates
Cell growth
Chromosome Mapping
Coliphages
Diploidy
Enzyme Repression
Enzymes
Escherichia coli
Escherichia coli - enzymology
Escherichia coli - growth & development
Escherichia coli - metabolism
Genes
Genes, Dominant
Genes, Recessive
Genetic mutation
Genotype
Genotypes
Heterozygote
Imidazoles
Ligases - analysis
Minerals
Mutagenesis
Mutation
Recombination, Genetic
Repression
Transduction, Genetic
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Summary:A recessive mutation (bir) of E. coli, at 77 min on the standard map, destroys ability of the bacteria to take up biotin or to grow except at high concentrations of biotin. In a biotin prototroph, the bir mutation causes overproduction and excretion of biotin, and derepression of at least one biosynthetic gene (bioD). Growth of the bir mutant is inhibited by avidin or high concentrations of dethiobiotin. Both effects are reversed by biotin.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.69.3.676