Fatty acid accumulation in the yeast Sporidiobolus salmonicolor during batch production of γ-decalactone

• Published in: FEMS microbiology letters Vol. 149; no. 1; pp. 17 - 24
• Main Authors: Feron, Gilles, Dufossé, Laurent, Mauvais, Geneviève, Bonnarme, Pascal, Spinnler, Henry-Eric
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 01.04.1997
• Subjects: Biological Sciences; Fatty Acids - metabolism; Fungal Proteins - chemistry; Fungal Proteins - metabolism; Lactone; Lactones - chemistry; Lactones - metabolism; Microbiological Techniques; Microscopy, Electron; Oleic Acid - metabolism; Oleic Acid - pharmacology; plant biochemistry; plant physiology; Ricinoleic acid; Ricinoleic Acids - metabolism; Ricinoleic Acids - pharmacology; Sporidiobolus; Sporidiobolus salmonicolor; Toxicity; Yeasts - drug effects; Yeasts - metabolism; Yeasts - ultrastructure; Lactone; Ricinoleic acid; Sporidiobolus; Toxicity
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1016/S0378-1097(97)00048-7

This paper provides new information about the metabolism of various fatty acids and γ-decalactone production by yeast. An analysis of the fatty acid composition of the yeast Sporidiobolus salmonicolor during batch production of lactone with ricinoleic acid methyl ester as a precursor showed an accumulation of the γ-decalactone precursor inside the cells. Electron microscopy of the yeasts showed the presence of large internal inclusions leading to membrane and organelle lysis and, consequently, death of the yeast. S. salmonicolor cultivated with methyl oleate did not produce γ-decalactone and is viable during the whole culture. Analysis of the long chain fatty acid fraction showed incorporation of methyl oleate.