Metallomic study on plasma samples from Nile tilapia using SR-XRF and GFAAS after separation by 2D PAGE: initial results

• Published in: Mikrochimica acta (1966) Vol. 173; no. 1-2; pp. 43 - 49
• Main Authors: Santos, Felipe A., Lima, Paula M., Neves, Renato C. F., Moraes, Paula M., Pérez, Carlos A., Silva, Marcelo O. A., Arruda, Marco A. Z., Castro, Gustavo R., Padilha, Pedro de Magalhães
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.04.2011; Springer
• Subjects: Analytical Chemistry; Blood proteins; Brackish; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Ethylenediaminetetraacetic acid; Exact sciences and technology; Fishes; Manganese; Microengineering; Nanochemistry; Nanotechnology; Oreochromis niloticus; Original Paper; Protein binding; Proteins; Separation; Spectrometric and optical methods; Spots; Strontium; Tilapia; Two dimensional; X-ray spectroscopy; Zinc; 2D PAGE; Nile tilapia; SR-XRF; Metalloproteins; GFAAS; Plasma; Graphite furnace; Sample; Concentration; Zinc; Isoelectric point; Acid digestion; Thermoelectric atomization; X ray fluorescence spectrometry; Molecular mass; Synchrotron radiation; Manganese; G protein; Atomic absorption spectrometry
• ISSN: 0026-3672; 1436-5073
• DOI: 10.1007/s00604-010-0522-y

An investigation was made on plasma samples obtained after protein separation. The proteome of the plasma of Nile tilapia ( Oreochromis niloticus ) was separated by 2D PAGE, and manganese and zinc in protein spots was qualitatively and quantitatively determined by synchrotron radiation X-ray fluorescence (SR-XRF) and graphite furnace atomic absorption spectrometry (GFAAS). Manganese and zinc are present in four and six plasma protein spots, respectively. These ions are bound to proteins with molecular weights ranging from 19 to 70 kDa and with isoelectric point (pI) ranging from 4.7 to 6.3. The concentrations of manganese and zinc bound to these proteins as determined by GFAAS following acid digestion of the spots range from 0.8 to 2.6 mg of manganese, and from 1.0 to 6.3 mg of zinc, respectively, per g of protein. Figure 2D-Page Plasma