Facilely prepared low-density DNA monolayer–based electrochemical biosensor with high detection performance in human serum

Presently, most reported electrochemical biosensors, for highly sensitive and selective detection of nucleic acid, still require multiple, time-consuming assembly steps and high-consumption DNA probes as well as lack good performance in human serum, which greatly limit their applicability. Herein, a...

Full description

Saved in:
Bibliographic Details
Published inAnalytical and bioanalytical chemistry Vol. 411; no. 10; pp. 2101 - 2109
Main Authors Chen, Jinyuan, Ye, Chenliu, Liu, Zhoujie, Yang, Liangyong, Liu, Ailin, Zhong, Guangxian, Peng, Huaping, Lin, Xinhua
Format Journal Article
LanguageEnglish
Published Berlin/Heidelberg Springer Berlin Heidelberg 01.04.2019
Springer
Springer Nature B.V
Subjects
Amplification
Analysis
Analytical Chemistry
Assembly
Background noise
Biochemistry
Biosensors
Blood serum
Bovine serum albumin
Catalysis
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Density
Deoxyribonucleic acid
DNA
DNA probes
Electrochemical analysis
Food Science
Human performance
Laboratory Medicine
Methods
Monitoring/Environmental Analysis
Monolayers
Nucleic acids
Research Paper
Serum albumin
Steric hindrance
Isothermal amplification
Low-density DNA monolayer
Steric hindrance effect
Electrochemical DNA biosensor
Online AccessGet full text

Cover

More Information
Summary:Presently, most reported electrochemical biosensors, for highly sensitive and selective detection of nucleic acid, still require multiple, time-consuming assembly steps and high-consumption DNA probes as well as lack good performance in human serum, which greatly limit their applicability. Herein, an easy-to-fabricate electrochemical DNA biosensor constructed by assembly of bovine serum albumin (BSA) followed with direct incubation of amplified products has been proposed. This method combined terminal deoxynucleoside transferase (TdTase)–mediated isothermal amplification and polyHRP catalysis to achieve dual-signal enhancement, and was featured with low-density DNA monolayer for its employment of only 2 nM capture probes. Surprisingly, based on the low-density DNA monolayer, the steric hindrance effect of polyHRP could effectively restrain the background compared with HRP, which further pushes the signal-to-noise (S/N) ratio to 70 than that of most currently available methods. Additionally, this strategy also showed favorable specificity and powerful anti-interference in human serum, and thus potentially attractive for diagnosis of diseases.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-019-01637-0