Influence of nonylphenol from multilayer plastic films on artificial insemination of sows

• Published in: Analytical and bioanalytical chemistry Vol. 412; no. 24; pp. 6519 - 6528
• Main Authors: Nerín, Cristina, Su, Qi-Zhi, Vera, Paula, Mendoza, Noelia, Ausejo, Raquel
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.09.2020; Springer; Springer Nature B.V
• Subjects: Acrosomes; Agricultural practices; Analytical Chemistry; Animals; Artificial insemination; Biochemistry; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Ethanol; Female; Female Role Models in Analytical Chemistry; Fertility; Fertility - drug effects; Food Science; Insemination, Artificial; Isomers; Laboratory Medicine; Leaching; Livestock; Livestock farming; Male; Migrants; Mitochondria; Mode of action; Monitoring/Environmental Analysis; Multilayers; Nonylphenol; Phenols - adverse effects; Phenols - analysis; Plastics; Plastics - adverse effects; Plastics - chemistry; Polymer films; Reproduction (biology); Research Paper; Semen; Semen - cytology; Semen - drug effects; Semen Analysis; Sperm; Swine - physiology; Spain; Semen plastic bags; Reprotoxicity; Artificial insemination; Non-target analysis; Migration from plastics
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-020-02698-2

Artificial insemination is common practice in mass livestock farming. Recently, it was shown that chemicals leaching from multilayer plastic bags affect the fertility of boars, although common quality tests did not show any impact on the sperm. It is not clear whether this incidence was a single case or whether it could be a systematic problem. Therefore, we studied six multilayer plastic bags. A total of 49 compounds were found, but most of them were at very low intensity. Nonylphenols in the range of 19-99 μg/g plastic were found. Migration tests using water and 10% ethanol as simulants, to mimic the behavior of semen with the extender, were performed. The most interesting migrants in terms of potential reprotoxicity were identified as nonylphenols. The identification in depth demonstrated the presence of 10 isomers of nonylphenol with a total concentration range between 16 to 58 μg/Kg simulant, among other migrants at lower concentration. The influence of these nonylphenols and their maximum tolerable concentration in direct contact with semen from boars was studied. Motility, viability, mitochondrial activity and acrosomes reacted were significantly affected at 10 mg/Kg of nonylphenols in contact with the sperm, but in vitro penetration rate was significantly decreased with only 2 mg/Kg. Insight into the mode of action is also provided.