Joint effects of eNOS gene T-786C and ADH2 Arg47His polymorphisms on the risk of premature coronary artery disease

• Published in: Thrombosis research Vol. 120; no. 5; pp. 679 - 684
• Main Authors: Jia, Chongqi, Liu, Tongtao, Liu, Zhaolan, Li, Min, Hu, Maohong
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Ltd 2007; Elsevier Science
• Subjects: Adult; Alcohol dehydrogenase; Alcohol Dehydrogenase - genetics; Arginine - chemistry; Atherosclerosis (general aspects, experimental research); Biological and medical sciences; Blood and lymphatic vessels; Cardiology. Vascular system; Case-Control Studies; China; Coronary artery disease; Coronary Artery Disease - ethnology; Coronary Artery Disease - genetics; Coronary heart disease; Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous; Endothelial nitric oxide synthase; Female; Gene; Genotype; Heart; Hematology, Oncology and Palliative Medicine; Histidine - chemistry; Humans; Interaction; Male; Medical sciences; Middle Aged; Nitric Oxide Synthase Type III - genetics; Polymorphism; Polymorphism, Genetic; Risk; China; coronary artery disease; triglyceride; NO; eNOS; ADH; alcohol dehydrogenase; odds ratio; RERI; systolic blood pressure; total cholesterol; DBP; S; diastolic blood pressure; Gene; synergy index; endothelial nitric oxide synthase; BMI; SBP; nitric oxide; percutaneous coronary angiography; OR; CI; body mass index; Interaction; CAD; relative excess risk due to interaction; PCA; TC; AP; TG; coronary heart disease; confidence interval; attributable proportion due to interaction; CHD; Polymorphism; Alcohol dehydrogenase; Coronary artery disease; Endothelial nitric oxide synthase; Human; Premature; Enzyme; Genotype; Cardiovascular disease; Case control study; Joint; Coronary heart disease; Nitric-oxide synthase; Osteoarticular system; Polymerase chain reaction; Logistic regression; Restriction fragment length polymorphism; Risk factor; Chinese; Oxidoreductases; Mutation
• ISSN: 0049-3848; 1879-2472
• DOI: 10.1016/j.thromres.2006.12.023

Abstract Introduction Both T-786C mutation in endothelial nitric oxide synthase (eNOS) gene and alcohol dehydrogenase (ADH) gene polymorphism such as ADH3 γ1/γ2 have been reportedly associated with coronary artery disease (CAD). Since ADH2 Arg47His polymorphism is common in Asian population, the aim of this present study was to assess the interaction between eNOS gene T-786C and ADH2 Arg47His polymorphisms on premature CAD risk. Materials and methods Hospital-based case–control study was conducted with 167 premature CAD and 235 late-onset CAD patients. Polymerase chain reaction restriction fragment length polymorphism was used to detect the polymorphisms. Multivariate logistic regression model was performed to adjust the potential confounders and estimate odds ratios (ORs) with 95% confidence intervals (CIs). Synergy index (S) was the measure to assess the interaction as departure from additivity. Results After the adjustment for the potential confounders, and compared with the carriers of TT and Arg/Arg as the reference, the ORs with 95% CIs in parentheses of premature CAD were that 1.13 (0.19–6.59) for CT or CC and Arg/Arg carriers; 2.24 (0.77–6.49) for TT and Arg/His or His/His carriers; 4.18 (1.32–13.22) for CT or CC and Arg/His or His/His carriers, respectively. Based on those ORs, S was 2.32 (95% CI: 0.37–14.72). Conclusions The mutant genotypes of eNOS gene T-786C mutation and the fast form of ADH2 Arg47His polymorphism had an additive interaction on the risk of premature CAD in Chinese population. Further investigations with big sample size are necessary for confirming this additive interaction.