Valproic Acid Induces CYP3A4 and MDR1 Gene Expression by Activation of Constitutive Androstane Receptor and Pregnane X Receptor Pathways

• Published in: Drug metabolism and disposition Vol. 35; no. 7; pp. 1032 - 1041
• Main Authors: CERVENY, Lukas, SVECOVA, Lucie, ANZENBACHEROVA, Eva, VRZAL, Radim, STAUD, Frantisek, DVORAK, Zdenek, ULRICHOVA, Jitka, ANZENBACHER, Pavel, PAVEK, Petr
• Format: Journal Article
• Language: English
• Published: Bethesda, MD American Society for Pharmacology and Experimental Therapeutics 01.07.2007
• Subjects: Anticonvulsants - pharmacology; Aryl Hydrocarbon Hydroxylases - genetics; ATP Binding Cassette Transporter, Subfamily B, Member 1 - biosynthesis; ATP Binding Cassette Transporter, Subfamily B, Member 1 - genetics; Biological and medical sciences; Cell Line, Tumor; Cytochrome P-450 CYP2B6; Cytochrome P-450 CYP3A; Cytochrome P-450 Enzyme System - biosynthesis; Cytochrome P-450 Enzyme System - genetics; Drug Synergism; Electrophoretic Mobility Shift Assay; Enzyme Induction; Genes, Reporter; Hepatocytes - drug effects; Hepatocytes - enzymology; Hepatocytes - metabolism; Humans; Hydroxylation; Luciferases; Medical sciences; Oxidoreductases, N-Demethylating - genetics; Oximes - pharmacology; Pharmacology. Drug treatments; Pregnane X Receptor; Promoter Regions, Genetic - drug effects; Receptors, Cytoplasmic and Nuclear - drug effects; Receptors, Cytoplasmic and Nuclear - metabolism; Receptors, Steroid - drug effects; Receptors, Steroid - metabolism; Retinoid X Receptor alpha - drug effects; Retinoid X Receptor alpha - metabolism; Reverse Transcriptase Polymerase Chain Reaction; Rifampin - pharmacology; RNA, Messenger - metabolism; Testosterone - metabolism; Thiazoles - pharmacology; Transcription Factors - drug effects; Transcription Factors - metabolism; Transcription, Genetic - drug effects; Transcriptional Activation - drug effects; Transfection; Up-Regulation; Valproic Acid - pharmacology; 4-Aminobutyrate transaminase; Enzyme; Isozyme; Transferases; Cytochrome P450; Pregnane X receptor; Enzyme inhibitor; Anticonvulsant; Valproic acid; Gene expression; Mood stabilizer; Transaminases; MDR-1 gene; Androstane; Biological receptor
• ISSN: 0090-9556; 1521-009X
• DOI: 10.1124/dmd.106.014456

In our study, we tested the hypothesis whether valproic acid (VPA) in therapeutic concentrations has potential to affect expression of CYP3A4 and MDR1 via constitutive androstane receptor (CAR) and pregnane X receptor (PXR) pathways. Interaction of VPA with CAR and PXR nuclear receptors was studied using luciferase reporter assays, real-time reverse transcriptase polymerase chain reaction (RT-PCR), electrophoretic mobility shift assay (EMSA), and analysis of CYP3A4 catalytic activity. Using transient transfection reporter assays in HepG2 cells, VPA was recognized to activate CYP3A4 promoter via CAR and PXR pathways. By contrast, a significant effect of VPA on MDR1 promoter activation was observed only in CAR-cotransfected HepG2 cells. These data well correlated with up-regulation of CYP3A4 and MDR1 mRNAs analyzed by real-time RT-PCR in cells transfected with expression vectors encoding CAR or PXR and treated with VPA. In addition, VPA significantly up-regulated CYP3A4 mRNA in primary hepatocytes and augmented the effect of rifampicin. EMSA experiments showed VPA-mediated augmentation of CAR/retinoid X receptor α heterodimer binding to direct repeat 3 (DR3) and DR4 responsive elements of CYP3A4 and MDR1 genes, respectively. Finally, analysis of specific CYP3A4 catalytic activity revealed its significant increase in VPA-treated LS174T cells transfected with PXR. In conclusion, we provide novel insight into the mechanism by which VPA affects gene expression of CYP3A4 and MDR1 genes. Our results demonstrate that VPA has potential to up-regulate CYP3A4 and MDR1 through direct activation of CAR and/or PXR pathways. Furthermore, we suggest that VPA synergistically augments the effect of rifampicin in transactivation of CYP3A4 in primary human hepatocytes.