Aerobic biodegradation characteristics and metabolic products of quinoline by a Pseudomonas strain

• Published in: Bioresource technology Vol. 100; no. 21; pp. 5030 - 5036
• Main Authors: Sun, Qinghua, Bai, Yaohui, Zhao, Cui, Xiao, Yana, Wen, Donghui, Tang, Xiaoyan
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.11.2009; [New York, NY]: Elsevier Ltd; Elsevier
• Subjects: Aerobiosis - drug effects; Ammonia - analysis; Biodegradation; Biodegradation, Environmental - drug effects; Biological and medical sciences; Biotechnology; Culture Media; Fundamental and applied biological sciences. Psychology; Gas Chromatography-Mass Spectrometry; Genes, Bacterial; Hydrogen-Ion Concentration - drug effects; Inorganic Chemicals - analysis; Metabolic products; Nitrogen - analysis; Nitrogen transformation; Organic Chemicals - analysis; Plasmids - isolation & purification; Polymerase Chain Reaction; Pseudomonas; Pseudomonas - drug effects; Pseudomonas - genetics; Pseudomonas - isolation & purification; Pseudomonas - metabolism; Pseudomonas sp; Quinoline; Quinolines - metabolism; Quinolines - pharmacology; Temperature; Biodegradation; Quinoline; Nitrogen transformation; Pseudomonas sp; Metabolic products; Pseudomonadales; Chemical transformation; Aerobe; Nitrogen; Metabolism; Pseudomonas; Bacteria; Pseudomonadaceae
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2009.05.044

A bacterial strain, BW003, which utilized quinoline as its sole C, N and energy source, was isolated and identified as Pseudomonas sp. BW003 degraded 192–911 mg/l quinoline within 3–8 h with removal rates ranging from 96% to 98%. The optimum conditions for the degradation were 30 °C and pH 8. In the process of biodegradation, at least 43% of quinoline was transformed into 2-hydroxyquinoline, then 0.69% of 2-hydroxyquinoline was transformed into 2,8-dihydroxyquinoline, and then, presumably, into 8-hydroxycoumarin. Meanwhile, at least 48% of the nitrogen in quinoline was directly transformed into ammonia-N. An extra carbon source enhanced the nitrogen transformation from ammonia-N. Further experiments showed that, besides cell synthesis, BW003 transformed less than 6% of ammonia-N into nitrate through heterotrophic nitrification. In addition, BW003 contained a large plasmid, which may be involved in quinoline metabolism. The study indicates that quinoline and its metabolic products can be eliminated from wastewater by controlling the C/N ratio using BW003 as the bioaugmentation inoculum.