Physio-biochemical and transcriptomic analysis reveals that the mechanism of Bacillus cereus G2 alleviated oxidative stress of salt-stressed Glycyrrhiza uralensis Fisch. seedlings

• Published in: Ecotoxicology and environmental safety Vol. 247; p. 114264
• Main Authors: Wang, Qiuli, Peng, Xueying, Lang, Duoyong, Ma, Xin, Zhang, Xinhui
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01.12.2022; Elsevier
• Subjects: Antioxidant enzymes; AsA-GSH cycle; Bacillus cereus G2; Glycyrrhiza uralensis Fisch; Osmo-protectants; Salt stress; O2; APX; G2; MDA; BM; AsA-GSH cycle; H2O2; ProDH; Osmo-protectants; GPX; P5Cs; INV; G. uralensis; GSH; BADH; Glycyrrhiza uralensis Fisch; MDHAR; SS; GSSG; Salt stress; POD; AsA-GSH; Antioxidant enzymes; SOD; DHAR; GR; AsA; ROS; CAT; DHA; Bacillus cereus G2
• ISSN: 0147-6513; 1090-2414; 1090-2414
• DOI: 10.1016/j.ecoenv.2022.114264

Salt stress severely affects the growth and productivity of Glycyrrhiza uralensis. Our previous research found that the endophyte Bacillus cereus G2 alleviated the osmotic and oxidative stress in G. uralensis exposed to salinity. However, the mechanism is still unclear. Here, a pot experiment was conducted to analyse the change in parameters related to osmotic adjustment and antioxidant metabolism by G2 in salt-stressed G. uralensis at the physio-biochemistry and transcriptome levels. The results showed that G2 significantly increased proline content by 48 %, glycine betaine content by 75 % due to activated expression of BADH1, and soluble sugar content by 77 % due to upregulated expression of α-glucosidase and SS, which might help to decrease the cell osmotic potential, enable the cell to absorb water, and stabilize the cell’s protein and membrane structure, thereby alleviating osmotic stress. Regarding antioxidant metabolism, G2 significantly decreased malondialdehyde (MDA) content by 27 %, which might be ascribed to the increase in superoxide dismutase (SOD) activity that facilitated the decrease in the superoxide radical (O2‾) production rate; it also increased the activities of catalase (CAT), ascorbate peroxidase (APX) and glutathione peroxidase (GPX), which helped stabilize the normal level of hydrogen peroxide (H2O2). G2 also increased glutathione (GSH) content by 65 % due to increased glutathione reductase (GR) activity and GSH/GSSG ratio, but G2 decreased oxidized glutathione (GSSG) content by 13 % due to decreased activity of dehydroascorbate reductase (DHAR), which could provide sufficient substrates for the ascorbate-glutathione (AsA-GSH) cycle to eliminate excess H2O2 that was not cleared in a timely manner by the antioxidant enzyme system. Taken together, G2 alleviated osmotic stress by increasing proline, soluble sugar, and glycine betaine contents and alleviated oxidative stress by the synergistic effect of antioxidant enzymes and the AsA-GSH cycle. Therefore, the results may be useful for explaining the mechanism by which endophyte inoculation regulates the salt tolerance of crops. [Display omitted] •Plant endophyte G2 alleviated oxidative stress in salt-stressed G. uralensis.•G2 alleviated oxidative stress by the synergy of antioxidant enzymes, AsA-GSH cycle, glycine betaine and proline.•G2 increased the accumulation of proline, glycine betaine and soluble sugar, which reduced the cell osmotic potential.•G2 improved antioxidant enzymes activities and enhanced nonenzyme antioxidants accumulation, which eliminated excess ROS.