Analysis of the mechanism for enhanced pyrene biodegradation based on the interactions between iron-ions and Rhodococcus ruber strain L9

• Published in: Ecotoxicology and environmental safety Vol. 225; p. 112789
• Main Authors: Liu, Jing, Zhang, Ai-Ning, Liu, Yong-Jun, Liu, Zhe, Liu, Yu, Wu, Xi-Jun
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01.12.2021; Elsevier
• Subjects: Biodegradation; Iron ion - driven; Mechanism; Pyrene; Rhodococcus ruber strain L9; Biodegradation; Pyrene; Iron ion - driven; Mechanism; Rhodococcus ruber strain L9
• ISSN: 0147-6513; 1090-2414; 1090-2414
• DOI: 10.1016/j.ecoenv.2021.112789

A slow degradation rate and low transformation efficiency are the main problems in the biodegradation of polycyclic aromatic hydrocarbons (PAHs). This study selected pyrene as the target PAH to investigate the effect of ferrous ion and ferric ion on pyrene degradation. The driving effect and mechanism, including the interaction between pyrene and iron ions and the bacterial physiological response during the biodegradation process by Rhodococcus ruber strain L9, were investigated. The results showed that iron ions did not enhance bacterial growth but improved bacteria’s pyrene removal capacity, contributing to the total efficiency of pyrene biodegradation. The process started with an initial formation of “cation-π” between Fe (III) and pyrene, which subsequently drove the pyrene removal process and accelerated the bacterial metabolic process. Moreover, a significant increase in the protein concentration, catechol dioxygenase (C12O and C23O) activities, and intracellular protein regulation in crude enzyme solution indicate a positive response of the bacteria during the iron ion-enhanced pyrene degradation process. [Display omitted] •The addition of iron ions can significantly promote the biodegradation of pyrene.•The protein concentration and enzyme mass-specific activities were remarkably improved by iron ion amendment.•The interaction of pyrene with iron ions accelerates bacterial protein response.•The mechanism of iron ion amendment in pyrene biodegradation was explored.