Tau antigenic changes induced by glutamate in rat primary culture model: a biochemical approach

Primary neuronal cultures were treated with glutamate to induce an increase of Tau immunoreactivity similar to that observed in Alzheimer's disease. The Tau profile of neurones in culture before and after exposure to glutamate was analyzed on immunoblots with anti-Tau, anti-paired helical filam...

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Bibliographic Details
Published inNeuroscience letters Vol. 140; no. 2; p. 206
Main Authors Sautière, P E, Sindou, P, Couratier, P, Hugon, J, Wattez, A, Delacourte, A
Format Journal Article
LanguageEnglish
Published Ireland 22.06.1992
Subjects
Alzheimer Disease - pathology
Animals
Cells, Cultured
Glutamates - toxicity
Glutamic Acid
Immunoblotting
Neurons - drug effects
Rats
tau Proteins - drug effects
tau Proteins - immunology
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Summary:Primary neuronal cultures were treated with glutamate to induce an increase of Tau immunoreactivity similar to that observed in Alzheimer's disease. The Tau profile of neurones in culture before and after exposure to glutamate was analyzed on immunoblots with anti-Tau, anti-paired helical filaments (PHF) and antibody specific for modified Tau. Differences were observed between treated and control cultures: glutamate induced a shift of immunodetection from the lowest to the highest molecular weight Tau isoform and an acidification of Tau proteins. However, these modifications are not exactly those observed in Alzheimer's disease since we were not able to detect 'Alzheimer-type' epitopes on Tau proteins after the glutamate exposure.
ISSN:0304-3940
DOI:10.1016/0304-3940(92)90104-F